Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers

Drabik, Dominik (avtor), Drab, Mitja (avtor), Penič, Samo (avtor), Iglič, Aleš (avtor), Czogalla, Aleksander (avtor)

Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present signifcant challenges. In this study we employ spotvariation z-scan fuorescence correlation spectroscopy (svzFCS) tailored for artifcial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of defnitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies—including the analysis of difusion coefcient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confrming that nano- and microdomains can co-exist in lipid vesicles.

• Jezik: Angleški jezik
• Ključne besede: lipid bilayers, microdomains, Monte Carlo simulations, ceramide 1 phosphate
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 1-14
• Številčenje: 13, article number 18570
• PID: 20.500.12556/RUL-155446
• UDK: 577
• ISSN pri članku: 2045-2322
• DOI: 10.1038/s41598-023-45575-5
• COBISS.SI-ID: 171022339
• Datum objave v RUL: 02.04.2024

Gradivo je del revije

• Naslov: Scientific reports
• Skrajšan naslov: Sci. rep.
• Založnik: Nature Publishing Group
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: lipidni dvosloj, mikrodomene, Monte Carlo simulacije, ceramid 1 fosfat