20.500.12556/RUL-155446 Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present signifcant challenges. In this study we employ spotvariation z-scan fuorescence correlation spectroscopy (svzFCS) tailored for artifcial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of defnitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies—including the analysis of difusion coefcient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confrming that nano- and microdomains can co-exist in lipid vesicles. lipid bilayers microdomains Monte Carlo simulations ceramide 1 phosphate lipidni dvosloj mikrodomene Monte Carlo simulacije ceramid 1 fosfat true false true Angleški jezik Slovenski jezik Članek v reviji 2024-04-02 13:22:57 2024-04-02 13:22:58 2024-04-03 03:48:58 0000-00-00 00:00:00 2023 0 0 Str. 1-14 13, article number 18570 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2045-2322 10.1038/s41598-023-45575-5 171022339 s41598-023-45575-5.pdf s41598-023-45575-5.pdf 1 C2963DE64BAA85D2EBD5A6C399ADEC5A 2d2d3f37caf6da47ccf45a7610769a3ece65d3045f5d420cee858a99ab1a8978 14c0a50f-f0e4-11ee-87d4-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182335 https://www.nature.com/articles/s41598-023-45575-5 1 5c2fadf8-f0e3-11ee-87d4-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182333 Fakulteta za elektrotehniko 0 0 0