Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers

Drabik, Dominik (author), Drab, Mitja (author), Penič, Samo (author), Iglič, Aleš (author), Czogalla, Aleksander (author)

Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present signifcant challenges. In this study we employ spotvariation z-scan fuorescence correlation spectroscopy (svzFCS) tailored for artifcial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of defnitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies—including the analysis of difusion coefcient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confrming that nano- and microdomains can co-exist in lipid vesicles.

• Language: English
• Keywords: lipid bilayers, microdomains, Monte Carlo simulations, ceramide 1 phosphate
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: Str. 1-14
• Numbering: 13, article number 18570
• PID: 20.500.12556/RUL-155446
• UDC: 577
• ISSN on article: 2045-2322
• DOI: 10.1038/s41598-023-45575-5
• COBISS.SI-ID: 171022339
• Publication date in RUL: 02.04.2024

DRABIK, Dominik, DRAB, Mitja, PENIČ, Samo, IGLIČ, Aleš and CZOGALLA, Aleksander, 2023, Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers. Scientific reports [online]. 2023. Vol. 13,  number 18570, p. 1–14. [Accessed 11 April 2025]. DOI 10.1038/s41598-023-45575-5. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=155446

Record is a part of a journal

• Title: Scientific reports
• Shortened title: Sci. rep.
• Publisher: Nature Publishing Group
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: lipidni dvosloj, mikrodomene, Monte Carlo simulacije, ceramid 1 fosfat