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Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor
ID Menegatti, Tadej (Avtor), ID Žnidaršič Plazl, Polona (Avtor)

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Izvleček
A microbioreactor was developed in which selected amine transaminase was immobilized together with the cofactor pyridoxal phosphate (PLP) to allow efficient continuous transamination. The enzyme and cofactor were retained in a porous copolymeric hydrogel matrix formed in a two-plate microreactor with an immobilization efficiency of over 97%. After 10 days of continuous operation, 92% of the initial productivity was retained and no leaching of PLP or enzyme from the hydrogel was observed. The microbioreactor with co-immobilized cofactor showed similar performance with and without the addition of exogenous PLP, suggesting that the addition of PLP is not required during the process. The space-time yield of the microbioreactor was 19.91 g L−1 h−1, while the highest achieved biocatalyst productivity was 5.4 mg mgenzyme−1 h−1. The immobilized enzyme also showed better stability over a wider pH and temperature range than the free enzyme. Considering the time and cost efficiency of the immobilization process and the possibility of capacity expansion, such a system is of great potential for industrial application.

Jezik:Angleški jezik
Ključne besede:cofactor co-immobilization, hydrogel, microreactor, amine transaminase, continuous process
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Leto izida:2021
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Datum objave:04.11.2021
Št. strani:Str. 1-10
Številčenje:Vol. 9
PID:20.500.12556/RUL-136179 Povezava se odpre v novem oknu
UDK:66.098
ISSN pri članku:2296-4185
DOI:10.3389/fbioe.2021.752064 Povezava se odpre v novem oknu
COBISS.SI-ID:83665411 Povezava se odpre v novem oknu
Datum objave v RUL:15.04.2022
Število ogledov:84
Število prenosov:18
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Frontiers in bioengineering and biotechnology
Skrajšan naslov:Front. bioeng. biotechnol.
Založnik:Frontiers Media
ISSN:2296-4185
COBISS.SI-ID:523093017 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:04.11.2021

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:koimobilizacija kofaktorja, hidrogel, mikroreaktor, amin transaminaza, kontinuirni procesi

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P2-0191
Naslov:Kemijsko inženirstvo

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:J4-1775
Naslov:Razvoj mikropretočnih sistemov za analizo, izbor in uporabo bakterijskih celic

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:MR-51842

Financer:EC - European Commission
Program financ.:H2020
Številka projekta:811040
Naslov:Chair Of Micro Process Engineering and TEchnology
Akronim:COMPETE

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