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Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor
ID
Menegatti, Tadej
(
Avtor
),
ID
Žnidaršič Plazl, Polona
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,35 MB)
MD5: EBB057E15766C3C5534AD3BE12D5E275
URL - Izvorni URL, za dostop obiščite
https://www.frontiersin.org/articles/10.3389/fbioe.2021.752064/full
Galerija slik
Izvleček
A microbioreactor was developed in which selected amine transaminase was immobilized together with the cofactor pyridoxal phosphate (PLP) to allow efficient continuous transamination. The enzyme and cofactor were retained in a porous copolymeric hydrogel matrix formed in a two-plate microreactor with an immobilization efficiency of over 97%. After 10 days of continuous operation, 92% of the initial productivity was retained and no leaching of PLP or enzyme from the hydrogel was observed. The microbioreactor with co-immobilized cofactor showed similar performance with and without the addition of exogenous PLP, suggesting that the addition of PLP is not required during the process. The space-time yield of the microbioreactor was 19.91 g L−1 h−1, while the highest achieved biocatalyst productivity was 5.4 mg mgenzyme−1 h−1. The immobilized enzyme also showed better stability over a wider pH and temperature range than the free enzyme. Considering the time and cost efficiency of the immobilization process and the possibility of capacity expansion, such a system is of great potential for industrial application.
Jezik:
Angleški jezik
Ključne besede:
cofactor co-immobilization
,
hydrogel
,
microreactor
,
amine transaminase
,
continuous process
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Datum objave:
04.11.2021
Leto izida:
2021
Št. strani:
Str. 1-10
Številčenje:
Vol. 9
PID:
20.500.12556/RUL-136179
UDK:
66.098
ISSN pri članku:
2296-4185
DOI:
10.3389/fbioe.2021.752064
COBISS.SI-ID:
83665411
Datum objave v RUL:
15.04.2022
Število ogledov:
779
Število prenosov:
108
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Objavi na:
Gradivo je del revije
Naslov:
Frontiers in bioengineering and biotechnology
Skrajšan naslov:
Front. bioeng. biotechnol.
Založnik:
Frontiers Media
ISSN:
2296-4185
COBISS.SI-ID:
523093017
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
04.11.2021
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
koimobilizacija kofaktorja
,
hidrogel
,
mikroreaktor
,
amin transaminaza
,
kontinuirni procesi
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P2-0191
Naslov:
Kemijsko inženirstvo
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J4-1775
Naslov:
Razvoj mikropretočnih sistemov za analizo, izbor in uporabo bakterijskih celic
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:
Young researchers
Financer:
EC - European Commission
Program financ.:
H2020
Številka projekta:
811040
Naslov:
Chair Of Micro Process Engineering and TEchnology
Akronim:
COMPETE
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