20.500.12556/RUL-136179 Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor A microbioreactor was developed in which selected amine transaminase was immobilized together with the cofactor pyridoxal phosphate (PLP) to allow efficient continuous transamination. The enzyme and cofactor were retained in a porous copolymeric hydrogel matrix formed in a two-plate microreactor with an immobilization efficiency of over 97%. After 10 days of continuous operation, 92% of the initial productivity was retained and no leaching of PLP or enzyme from the hydrogel was observed. The microbioreactor with co-immobilized cofactor showed similar performance with and without the addition of exogenous PLP, suggesting that the addition of PLP is not required during the process. The space-time yield of the microbioreactor was 19.91 g L−1 h−1, while the highest achieved biocatalyst productivity was 5.4 mg mgenzyme−1 h−1. The immobilized enzyme also showed better stability over a wider pH and temperature range than the free enzyme. Considering the time and cost efficiency of the immobilization process and the possibility of capacity expansion, such a system is of great potential for industrial application. cofactor co-immobilization hydrogel microreactor amine transaminase continuous process koimobilizacija kofaktorja hidrogel mikroreaktor amin transaminaza kontinuirni procesi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-15 08:32:00 2022-04-15 08:32:02 2023-03-21 09:41:59 0000-00-00 00:00:00 2021 0 0 Str. 1-10 Vol. 9 4 Nov. 2021 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2021-11-04 66.098 2296-4185 10.3389/fbioe.2021.752064 83665411 Hydrogel_-_Polona_Plazl.pdf Hydrogel_-_Polona_Plazl.pdf 1 EBB057E15766C3C5534AD3BE12D5E275 53f4156cbc52d26a4aa0b6b2045cbcd08faecbbf9659ace2aac5126a095b8ea3 13f5340c-bc86-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155684 https://www.frontiersin.org/articles/10.3389/fbioe.2021.752064/full 1 b45cba1f-bc85-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155682 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0