Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor

Menegatti, Tadej (author), Žnidaršič Plazl, Polona (author)

A microbioreactor was developed in which selected amine transaminase was immobilized together with the cofactor pyridoxal phosphate (PLP) to allow efficient continuous transamination. The enzyme and cofactor were retained in a porous copolymeric hydrogel matrix formed in a two-plate microreactor with an immobilization efficiency of over 97%. After 10 days of continuous operation, 92% of the initial productivity was retained and no leaching of PLP or enzyme from the hydrogel was observed. The microbioreactor with co-immobilized cofactor showed similar performance with and without the addition of exogenous PLP, suggesting that the addition of PLP is not required during the process. The space-time yield of the microbioreactor was 19.91 g L−1 h−1, while the highest achieved biocatalyst productivity was 5.4 mg mgenzyme−1 h−1. The immobilized enzyme also showed better stability over a wider pH and temperature range than the free enzyme. Considering the time and cost efficiency of the immobilization process and the possibility of capacity expansion, such a system is of great potential for industrial application.

• Language: English
• Keywords: cofactor co-immobilization, hydrogel, microreactor, amine transaminase, continuous process
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Publication date: 04.11.2021
• Year: 2021
• Number of pages: Str. 1-10
• Numbering: Vol. 9
• PID: 20.500.12556/RUL-136179
• UDC: 66.098
• ISSN on article: 2296-4185
• DOI: 10.3389/fbioe.2021.752064
• COBISS.SI-ID: 83665411
• Publication date in RUL: 15.04.2022

Record is a part of a journal

• Title: Frontiers in bioengineering and biotechnology
• Shortened title: Front. bioeng. biotechnol.
• Publisher: Frontiers Media
• ISSN: 2296-4185
• COBISS.SI-ID: 523093017

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 04.11.2021

Secondary language

• Language: Slovenian
• Keywords: koimobilizacija kofaktorja, hidrogel, mikroreaktor, amin transaminaza, kontinuirni procesi