Development and optimisation of solid-phase extraction of extractable and bound phenolic acids in spelt (Triticum spelta L.) seeds

Mencin, Marjeta (avtor), Mikulič Petkovšek, Maja (avtor), Veberič, Robert (avtor), Terpinc, Petra (avtor)

A solid-phase extraction (SPE) technique was developed and optimised for isolation and concentration of extractable and bound phenolic acids from germinated spelt seeds, for analysis by liquid chromatography–mass spectrometry. Samples initially underwent solvent extraction under different conditions to maximise the yield of phenolic antioxidants. Optimal extraction conditions for extractable phenolics were absolute methanol as solvent, sample-to-methanol ratio 1:9, and reconstitution in non-acidified water. The bound phenolics were extracted from sample pellets using hydrolysis with 2 M NaOH, acidification of the hydrolysate with formic acid, and simultaneous isolation and purification using Strata X polymeric RP tubes. Compared to liquid-liquid extraction, this direct SPE protocol has significant advantages in terms of higher extraction efficiencies of total and individual phenolics and their antioxidant activities. These data suggest that direct SPE represents a rapid and reliable method for quantitative analysis of both the extractable and the commonly overlooked bound phenolics in Triticum spelta seeds.

• Jezik: Angleški jezik
• Ključne besede: spelt phenolics, extraction, hydrolysis, solid-phase extraction, liquid-liquid extraction, antioxidant activity, LC-MS
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 20 str.
• Številčenje: Vol. 10, iss. 7, art. 1085
• PID: 20.500.12556/RUL-135735
• UDK: 633.111.5:581.142:547.56
• ISSN pri članku: 2076-3921
• DOI: 10.3390/antiox10071085
• COBISS.SI-ID: 69476867
• Datum objave v RUL: 30.03.2022

Gradivo je del revije

• Naslov: Antioxidants
• Skrajšan naslov: Antioxidants
• Založnik: MDPI
• ISSN: 2076-3921
• COBISS.SI-ID: 522976025

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 05.07.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: pira, fenolne spojine, kalitev semen, antioksidativna aktivnost, ekstrakcija

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0234
• Naslov: Integrirano živilstvo in prehrana

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0013
• Naslov: Hortikultura