20.500.12556/RUL-135735 Development and optimisation of solid-phase extraction of extractable and bound phenolic acids in spelt (Triticum spelta L.) seeds A solid-phase extraction (SPE) technique was developed and optimised for isolation and concentration of extractable and bound phenolic acids from germinated spelt seeds, for analysis by liquid chromatography–mass spectrometry. Samples initially underwent solvent extraction under different conditions to maximise the yield of phenolic antioxidants. Optimal extraction conditions for extractable phenolics were absolute methanol as solvent, sample-to-methanol ratio 1:9, and reconstitution in non-acidified water. The bound phenolics were extracted from sample pellets using hydrolysis with 2 M NaOH, acidification of the hydrolysate with formic acid, and simultaneous isolation and purification using Strata X polymeric RP tubes. Compared to liquid-liquid extraction, this direct SPE protocol has significant advantages in terms of higher extraction efficiencies of total and individual phenolics and their antioxidant activities. These data suggest that direct SPE represents a rapid and reliable method for quantitative analysis of both the extractable and the commonly overlooked bound phenolics in Triticum spelta seeds. spelt phenolics extraction hydrolysis solid-phase extraction liquid-liquid extraction antioxidant activity LC-MS pira fenolne spojine kalitev semen antioksidativna aktivnost ekstrakcija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-30 09:21:51 2022-03-30 09:21:53 2022-09-14 04:14:54 0000-00-00 00:00:00 2021 0 0 20 str. iss. 7, art. 1085 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633.111.5:581.142:547.56 2076-3921 10.3390/antiox10071085 69476867 RAZ_Mencin_Marjeta_2021.pdf RAZ_Mencin_Marjeta_2021.pdf 1 10189FD11F800C9BA5BC7E62D5FAE05E 3386aa5d86fbc25dbe156e7938e977451e8d5d2d52b03cf0b5edb5fea1a6bc5d e77dea78-affc-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154998 https://www.mdpi.com/2076-3921/10/7/1085 1 0de829a9-affa-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154997 Biotehniška fakulteta 0 0 0