High-frequency and high-voltage asymmetric bipolar pulse generator for electroporation based technologies and therapies

Pirc, Eva (avtor), Miklavčič, Damijan (avtor), Uršič Valentinuzzi, Katja (avtor), Serša, Gregor (avtor), Reberšek, Matej (avtor)

Currently, in high-frequency electroporation, much progress has been made but limited to research groups with custom-made laboratory prototype electroporators. According to the review of electroporators and economic evaluations, there is still an area of pulse parameters that needs to be investigated. The development of an asymmetric bipolar pulse generator with a maximum voltage of 4 kV and minimum duration time of a few hundred nanoseconds, would enable in vivo evaluation of biological effects of high-frequency electroporation pulses. Herein, from a series of most commonly used drivers and optical isolations in high-voltage pulse generators the one with optimal characteristics was used. In addition, the circuit topology of the developed device is described in detail. The developed device is able to generate 4 kV pulses, with theoretical 131 A maximal current and 200 ns minimal pulse duration, the maximal pulse repetition rate is 2 MHz and the burst maximal repetition rate is 1 MHz. The device was tested in vivo. The effectiveness of electrochemotherapy of high-frequency electroporation pulses is compared to “classical” electrochemotherapy pulses. In vivo electrochemotherapy with high-frequency electroporation pulses was at least as effective as with “classical” well-established electric pulses, resulting in 86% and 50% complete responses, respectively. In contrast to previous reports, however, muscle contractions were comparable between the two protocols.

• Jezik: Angleški jezik
• Ključne besede: electroporation, electroporation device, high-frequency, bipolar, asymmetric, SiC MOSFET, high-voltage, pulse generator, electrochemotherapy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko; ZF - Zdravstvena fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 19 str.
• Številčenje: Vol. 10, iss. 10, art. 1203
• PID: 20.500.12556/RUL-135440
• UDK: 602.621:621.3
• ISSN pri članku: 2079-9292
• DOI: 10.3390/electronics10101203
• COBISS.SI-ID: 63928323
• Datum objave v RUL: 15.03.2022

Gradivo je del revije

• Naslov: Electronics
• Skrajšan naslov: Electronics
• Založnik: MDPI
• ISSN: 2079-9292
• COBISS.SI-ID: 523068953

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 18.05.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: elektroporacija, naprava za elektroporacijo, visoka frekvenca, bipolarni, asimetrični, SiC MOSFET, visoka napetost, pulzni generatorji, elektrokemoterapija