20.500.12556/RUL-135440 High-frequency and high-voltage asymmetric bipolar pulse generator for electroporation based technologies and therapies Currently, in high-frequency electroporation, much progress has been made but limited to research groups with custom-made laboratory prototype electroporators. According to the review of electroporators and economic evaluations, there is still an area of pulse parameters that needs to be investigated. The development of an asymmetric bipolar pulse generator with a maximum voltage of 4 kV and minimum duration time of a few hundred nanoseconds, would enable in vivo evaluation of biological effects of high-frequency electroporation pulses. Herein, from a series of most commonly used drivers and optical isolations in high-voltage pulse generators the one with optimal characteristics was used. In addition, the circuit topology of the developed device is described in detail. The developed device is able to generate 4 kV pulses, with theoretical 131 A maximal current and 200 ns minimal pulse duration, the maximal pulse repetition rate is 2 MHz and the burst maximal repetition rate is 1 MHz. The device was tested in vivo. The effectiveness of electrochemotherapy of high-frequency electroporation pulses is compared to “classical” electrochemotherapy pulses. In vivo electrochemotherapy with high-frequency electroporation pulses was at least as effective as with “classical” well-established electric pulses, resulting in 86% and 50% complete responses, respectively. In contrast to previous reports, however, muscle contractions were comparable between the two protocols. electroporation electroporation device high-frequency bipolar asymmetric SiC MOSFET high-voltage pulse generator electrochemotherapy elektroporacija naprava za elektroporacijo visoka frekvenca bipolarni asimetrični SiC MOSFET visoka napetost pulzni generatorji elektrokemoterapija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-15 09:41:43 2022-03-15 09:41:45 2022-09-14 04:13:20 0000-00-00 00:00:00 2021 0 0 19 str. iss. 10, art. 1203 Vol. 10 May 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621:621.3 2079-9292 10.3390/electronics10101203 63928323 RAZ_Pirc_Eva_2021.pdf RAZ_Pirc_Eva_2021.pdf 1 9000A6E9026E7E48A92726F373BF92B8 4b50f009975dbfacdd392451389d7e193b1378a3e5a954935e076947e32c4abb 05fd8745-a43c-11ec-8556-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154519 https://www.mdpi.com/2079-9292/10/10/1203 1 c2422c54-a43b-11ec-8556-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154516 Fakulteta za elektrotehniko Zdravstvena fakulteta 0 0 0