High-frequency and high-voltage asymmetric bipolar pulse generator for electroporation based technologies and therapies

Pirc, Eva (author), Miklavčič, Damijan (author), Uršič Valentinuzzi, Katja (author), Serša, Gregor (author), Reberšek, Matej (author)

Currently, in high-frequency electroporation, much progress has been made but limited to research groups with custom-made laboratory prototype electroporators. According to the review of electroporators and economic evaluations, there is still an area of pulse parameters that needs to be investigated. The development of an asymmetric bipolar pulse generator with a maximum voltage of 4 kV and minimum duration time of a few hundred nanoseconds, would enable in vivo evaluation of biological effects of high-frequency electroporation pulses. Herein, from a series of most commonly used drivers and optical isolations in high-voltage pulse generators the one with optimal characteristics was used. In addition, the circuit topology of the developed device is described in detail. The developed device is able to generate 4 kV pulses, with theoretical 131 A maximal current and 200 ns minimal pulse duration, the maximal pulse repetition rate is 2 MHz and the burst maximal repetition rate is 1 MHz. The device was tested in vivo. The effectiveness of electrochemotherapy of high-frequency electroporation pulses is compared to “classical” electrochemotherapy pulses. In vivo electrochemotherapy with high-frequency electroporation pulses was at least as effective as with “classical” well-established electric pulses, resulting in 86% and 50% complete responses, respectively. In contrast to previous reports, however, muscle contractions were comparable between the two protocols.

• Language: English
• Keywords: electroporation, electroporation device, high-frequency, bipolar, asymmetric, SiC MOSFET, high-voltage, pulse generator, electrochemotherapy
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering; ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 19 str.
• Numbering: Vol. 10, iss. 10, art. 1203
• PID: 20.500.12556/RUL-135440
• UDC: 602.621:621.3
• ISSN on article: 2079-9292
• DOI: 10.3390/electronics10101203
• COBISS.SI-ID: 63928323
• Publication date in RUL: 15.03.2022

Record is a part of a journal

• Title: Electronics
• Shortened title: Electronics
• Publisher: MDPI
• ISSN: 2079-9292
• COBISS.SI-ID: 523068953

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 18.05.2021

Secondary language

• Language: Slovenian
• Keywords: elektroporacija, naprava za elektroporacijo, visoka frekvenca, bipolarni, asimetrični, SiC MOSFET, visoka napetost, pulzni generatorji, elektrokemoterapija