Age-dependent expression of MyHC isoforms and lipid metabolism-related genes in the longissimus dorsi muscle of wild and domestic pigs

Vrecl, Milka (avtor), Cotman, Marko (avtor), Uršič, Matjaž (avtor), Čandek-Potokar, Marjeta (avtor), Fazarinc, Gregor (avtor)

This study aimed to compare age-dependent changes in the relative expression of genes encoding myosin heavy chain (MyHC) isoforms and selected lipid metabolism-related genes in the longissimus dorsi muscle of wild pigs (WPs) and domestic pigs (DPs). Muscles sampled from postnatal day one as well as three-week-old and two-year-old animals were used in quantitative polymerase chain reaction (qPCR) assays, histological evaluations of succinate dehydrogenase (SDH) activity, and intra-myofiber lipid (IMFL) assessment. Expression of the MyHC isoforms displayed the most extensive age- and breed-dependent changes within the first three postnatal weeks. The MyHCembry level decreased significantly faster in the WPs than in the DPs. The relative MyHC-I and -IIa expression was significantly higher in the WPs, and MyHC-IIb was substantially higher in the DPs. The differences in MyHC expression corroborated the number of SDH-positive myofibers and IMFLs. Expression of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase (LPL) genes displayed only age-related variations. In summary, the evidence is provided for accelerated postnatal myofiber transformation directed towards oxidative myofibers in WPs. The SDH activity/staining intensity largely reflected the expression of MyHCs, and not genes involved in lipid uptake and utilization.

• Jezik: Angleški jezik
• Ključne besede: swine, growth and development, myofibers, genetics, polymerase chain reaction, histocytochemistry, myosin heavy chains, isoforms, lipid metabolism, skeletal myofibers, postnatal transformation, quantitative PCR, histochemistry, MyHC isoforms, lipid metabolism-related genes
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: 12 str.
• Številčenje: Vol. 9, iss. 1, art. 10
• PID: 20.500.12556/RUL-108800
• UDK: 636.4.09:575:611.7
• ISSN pri članku: 2076-2615
• DOI: 10.3390/ani9010010
• COBISS.SI-ID: 4739194
• Datum objave v RUL: 26.07.2019

Gradivo je del revije

• Naslov: Animals
• Skrajšan naslov: Animals
• Založnik: MDPI AG
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.01.2019

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0053
• Naslov: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0133
• Naslov: Trajnostno kmetijstvo