20.500.12556/RUL-108800 Age-dependent expression of MyHC isoforms and lipid metabolism-related genes in the longissimus dorsi muscle of wild and domestic pigs This study aimed to compare age-dependent changes in the relative expression of genes encoding myosin heavy chain (MyHC) isoforms and selected lipid metabolism-related genes in the longissimus dorsi muscle of wild pigs (WPs) and domestic pigs (DPs). Muscles sampled from postnatal day one as well as three-week-old and two-year-old animals were used in quantitative polymerase chain reaction (qPCR) assays, histological evaluations of succinate dehydrogenase (SDH) activity, and intra-myofiber lipid (IMFL) assessment. Expression of the MyHC isoforms displayed the most extensive age- and breed-dependent changes within the first three postnatal weeks. The MyHCembry level decreased significantly faster in the WPs than in the DPs. The relative MyHC-I and -IIa expression was significantly higher in the WPs, and MyHC-IIb was substantially higher in the DPs. The differences in MyHC expression corroborated the number of SDH-positive myofibers and IMFLs. Expression of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase (LPL) genes displayed only age-related variations. In summary, the evidence is provided for accelerated postnatal myofiber transformation directed towards oxidative myofibers in WPs. The SDH activity/staining intensity largely reflected the expression of MyHCs, and not genes involved in lipid uptake and utilization. swine growth and development myofibers genetics polymerase chain reaction histocytochemistry myosin heavy chains isoforms lipid metabolism skeletal myofibers postnatal transformation quantitative PCR histochemistry MyHC isoforms lipid metabolism-related genes true false true Angleški jezik Ni določen Članek v reviji 2019-07-26 13:54:20 2019-07-26 13:54:21 2022-08-20 03:48:38 0000-00-00 00:00:00 2019 0 0 12 str. iss. 1, art. 10 Vol. 9 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.4.09:575:611.7 2076-2615 10.3390/ani9010010 4739194 519120409 RAZ_Vrecl_Milka_2019.pdf RAZ_Vrecl_Milka_2019.pdf 1 00ACB41A077875AEC2792B6477DE514E 071e2966a2098b86410ebb5d851dc43988daa9b986a65dd56769a673c6834813 b570ad02-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141144 https://www.mdpi.com/2076-2615/9/1/10 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=119920 Veterinarska fakulteta 0 0 0