Age-dependent expression of MyHC isoforms and lipid metabolism-related genes in the longissimus dorsi muscle of wild and domestic pigs

Vrecl, Milka (author), Cotman, Marko (author), Uršič, Matjaž (author), Čandek-Potokar, Marjeta (author), Fazarinc, Gregor (author)

This study aimed to compare age-dependent changes in the relative expression of genes encoding myosin heavy chain (MyHC) isoforms and selected lipid metabolism-related genes in the longissimus dorsi muscle of wild pigs (WPs) and domestic pigs (DPs). Muscles sampled from postnatal day one as well as three-week-old and two-year-old animals were used in quantitative polymerase chain reaction (qPCR) assays, histological evaluations of succinate dehydrogenase (SDH) activity, and intra-myofiber lipid (IMFL) assessment. Expression of the MyHC isoforms displayed the most extensive age- and breed-dependent changes within the first three postnatal weeks. The MyHCembry level decreased significantly faster in the WPs than in the DPs. The relative MyHC-I and -IIa expression was significantly higher in the WPs, and MyHC-IIb was substantially higher in the DPs. The differences in MyHC expression corroborated the number of SDH-positive myofibers and IMFLs. Expression of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase (LPL) genes displayed only age-related variations. In summary, the evidence is provided for accelerated postnatal myofiber transformation directed towards oxidative myofibers in WPs. The SDH activity/staining intensity largely reflected the expression of MyHCs, and not genes involved in lipid uptake and utilization.

• Language: English
• Keywords: swine, growth and development, myofibers, genetics, polymerase chain reaction, histocytochemistry, myosin heavy chains, isoforms, lipid metabolism, skeletal myofibers, postnatal transformation, quantitative PCR, histochemistry, MyHC isoforms, lipid metabolism-related genes
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2019
• Number of pages: 12 str.
• Numbering: Vol. 9, iss. 1, art. 10
• PID: 20.500.12556/RUL-108800
• UDC: 636.4.09:575:611.7
• ISSN on article: 2076-2615
• DOI: 10.3390/ani9010010
• COBISS.SI-ID: 4739194
• Publication date in RUL: 26.07.2019

Record is a part of a journal

• Title: Animals
• Shortened title: Animals
• Publisher: MDPI AG
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.01.2019

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0053
• Name: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0133
• Name: Trajnostno kmetijstvo