Design and optimization of phospholipid vesicles for topical betamethasone dipropionate

Casula, Luca (avtor), Vitek, Mercedes (avtor), Zvonar Pobirk, Alenka (avtor), Gosenca Matjaž, Mirjam (avtor), Valenti, Donatella (avtor), Dupont, Aurélien (avtor), Caddeo, Carla (avtor)

Betamethasone dipropionate is a topical corticosteroid widely used to treat common dermatoses and skin disorders. However, the poor skin penetration of traditional Betamethasone dipropionate formulations can impair therapeutic outcomes. In the present study, betamethasone dipropionate was formulated in phospholipid vesicles to maximize its benefits and minimize its side effects. Liposomes and transfersomes were developed using the direct sonication method and thoroughly characterized by light scattering, cryo-transmission electron microscopy (TEM), small-angle x-ray scattering (SAXS), rheological analysis and skin permeation studies. Human skin epithelial-like cells were used to study how the phospholipid vesicles affected cell proliferation and morphology. Both the liposomes and transfersomes were nanosized, spherical and predominantly unilamellar, but the transfersomes were smaller, more homogeneous and stable on storage due to the presence of Tween® 80. The latter was also responsible for a higher viscosity, which decreased at 32°C, the temperature of the skin surface, as determined by rheological analysis. Furthermore, tests of the formulations in skin cells showed the absence of cytotoxicity and increased skin permeation. Phospholipid vesicles represent a promising approach for betamethasone dipropionate delivery, as they are characterized by high stability and cytocompatibility. These properties may improve the therapeutic outcomes and minimize the side effects of betamethasone dipropionate, encouraging further investigation to confirm the effective and safe use of betamethasone dipropionate nanoformulations on the skin.

• Jezik: Angleški jezik
• Ključne besede: betamethasone dipropionate, phospholipid vesicles, ultrastructural analysis, keratinocyte proliferation, delivery to the skin
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2026
• Št. strani: 11 str.
• Številčenje: art. 107363
• PID: 20.500.12556/RUL-179776
• UDK: 66.017-022.513.2:616-097
• ISSN pri članku: 1879-0720
• DOI: 10.1016/j.ejps.2025.107363
• COBISS.SI-ID: 255995395
• Datum objave v RUL: 25.02.2026

Gradivo je del revije

• Naslov: European Journal of Pharmaceutical Sciences
• Založnik: Elsevier
• ISSN: 1879-0720
• COBISS.SI-ID: 23290117

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: betametazondipropionat, fosfolipidni vezikli, ultrastrukturna analiza, proliferacija keratinocitov, vnos skozi kožo, dermatološka zdravila, nanodelci, liposomi, fosfolipidi

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P1-0189
• Naslov: Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih