Callus-mediatede embryogenesis of leaf celery (Apium graveolens L. var. secalinum Alef.) as a response to malt, peptone and yeast extracts application

Girgis, Nancy Danial (avtor), Desoukey, Sally Farag (avtor), Amer, Ahmed Mohamed (avtor), Arafa, Nermeen Mohammad (avtor)

Long times ago, celery (Apium graveolens) of Apiaceae family is well-known traditional plant that possesses eminent therapeutic characteristics. The current work aims to develop an efficient protocol for celery embryogenesis via indirect callus cell cultures approach using biotic elicitors (malt, peptone and yeast) at different concentrations (1.5, 2.5, 3.5 g\l) of each. The optimal explant to induce calli was the stem explant on the best selected medium (0.5 mg/l 2, 4-D). Fresh weight and growth value of celery stem calli were progressively augmented even reach out to the highest values after 8 weeks of culture, whereas the highest growth rate was achieved after 5 weeks of culture. Malt extract (1.5 g/l) performed efficiently as the best biotic elicitor for superior value of callus fresh weight. Similarly, growth rate and growth value were enhanced to 1.2±0.009E-fg/day and 12.1±0.12F-e, respectively. Throughout the histological investigation, the anatomical structure for all elicited treatments of celery stem calli exhibited more potent efficiency of malt than peptone for embryogenesis development then the yeast extract. Meanwhile, free elicitors’ MS-medium stopped to the stage of globular embryo formation. This work authenticated the types and concentrations potency of elicitors for callusing augmentation and somatic embryogenesis development of Apium graveolens.

• Jezik: Angleški jezik
• Ključne besede: Apium graveolens L. var. secalinum, callus, embryogenesis, biotic elicitors, histological investigation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 12 str.
• Številčenje: Vol. 121, no. 4
• PID: 20.500.12556/RUL-177133
• UDK: 631
• ISSN pri članku: 1854-1941
• DOI: 10.14720/aas.2025.121.4.20659
• COBISS.SI-ID: 261721859
• Datum objave v RUL: 16.12.2025

Gradivo je del revije

• Naslov: Acta agriculturae Slovenica
• Založnik: Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Univerza v Ljubljani, Biotehniška fakulteta, Založba Univerze v Ljubljani
• ISSN: 1854-1941
• COBISS.SI-ID: 219581184

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Naslov: Embriogeneza listne zelene (Apium graveolens L. var. secalinum Alef.) iz kalusa z dodatki slada, peptona in izvlečkov kvasa
• Ključne besede: listna zelena, kalus, embriogeneza, biotski elicitorji, istološka preiskava