20.500.12556/RUL-177133 Callus-mediatede embryogenesis of leaf celery (Apium graveolens L. var. secalinum Alef.) as a response to malt, peptone and yeast extracts application Embriogeneza listne zelene (Apium graveolens L. var. secalinum Alef.) iz kalusa z dodatki slada, peptona in izvlečkov kvasa Long times ago, celery (Apium graveolens) of Apiaceae family is well-known traditional plant that possesses eminent therapeutic characteristics. The current work aims to develop an efficient protocol for celery embryogenesis via indirect callus cell cultures approach using biotic elicitors (malt, peptone and yeast) at different concentrations (1.5, 2.5, 3.5 g\l) of each. The optimal explant to induce calli was the stem explant on the best selected medium (0.5 mg/l 2, 4-D). Fresh weight and growth value of celery stem calli were progressively augmented even reach out to the highest values after 8 weeks of culture, whereas the highest growth rate was achieved after 5 weeks of culture. Malt extract (1.5 g/l) performed efficiently as the best biotic elicitor for superior value of callus fresh weight. Similarly, growth rate and growth value were enhanced to 1.2±0.009E-fg/day and 12.1±0.12F-e, respectively. Throughout the histological investigation, the anatomical structure for all elicited treatments of celery stem calli exhibited more potent efficiency of malt than peptone for embryogenesis development then the yeast extract. Meanwhile, free elicitors’ MS-medium stopped to the stage of globular embryo formation. This work authenticated the types and concentrations potency of elicitors for callusing augmentation and somatic embryogenesis development of Apium graveolens. Apium graveolens L. var. secalinum callus embryogenesis biotic elicitors histological investigation listna zelena kalus embriogeneza biotski elicitorji istološka preiskava true false true Angleški jezik Slovenski jezik Članek v reviji 2025-12-16 15:03:08 2025-12-16 15:03:15 2025-12-17 04:40:56 0000-00-00 00:00:00 2025 0 0 12 str. no. 4 Vol. 121 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 631 1854-1941 10.14720/aas.2025.121.4.20659 261721859 ARAFA+et+al.+(A).pdf ARAFA+et+al.+(A).pdf 1 CD4BCE6CBCDE5BE829CB64FAA9E6410D 32e7c2a017d25ca709faaa8c078c12b14b88f7799a10d35c48d37e893cbee911 eb6cdc38-da87-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=223985 https://journals.uni-lj.si/aas/article/view/20659/19766 1 87929607-da87-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=223984 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0