Lactococcus lactis as a delivery system for surface displayed mimotopes of major peanut allergen Ara h 2

Lunder, Mojca (avtor), Luzar, Jernej (avtor), Ključevšek, Tim (avtor), Berlec, Aleš (avtor), Štrukelj, Borut (avtor), Skerbinjek-Kavalar, Maja (avtor), Koren, Ana (avtor), Korošec, Peter (avtor)

The prevalence of peanut allergy has been steadily increasing over the last decades. Among food allergies it shows the highest severity and is potentially fatal. Allergen Ara h 2 stands out as the main elicitor of allergic reactions. Research in the field of peanut immunotherapy still strives to improve the significant drawbacks of currently approved therapeutics. In this study we tested three epitope-like peptides as epitope-specific treatment. The epitope-like peptides (mimotopes) of major peanut allergen Ara h 2 were expressed as fusions with scaffold protein – lactococcal peptidoglycan binding domain of AcmA. Mimotope-decorated L. lactis and mimotope-AcmA fusion proteins were characterized. The mimotopes retained the ability to bind IgE from peanut-allergic patients' sera. They showed low basophil activation in peanut-allergic patients, except for mimotope DHPRYGP fused to AcmA suggesting its allergenic activity was partially preserved due to the highest similarity with Ara h 2 epitope. Mimotopes fused to AcmA can elicit T cell response; the increased secretion of IFN-γ suggests the shift of T cell immune response to non-allergic type 1. The use of recombinant L. lactis as a delivery system for surface displayed mimotopes represents a promising strategy that would enable delivery and expression of mimotopes on mucosal surfaces and at the same time beneficial immunomodulating properties.

• Jezik: Angleški jezik
• Ključne besede: peanut allergy, Ara h 2, Lactococcus lactis, mimotopes
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 8 str.
• Številčenje: Vol. 543, art. 113921
• PID: 20.500.12556/RUL-175626
• UDK: 615.015.14:616-056.43
• ISSN pri članku: 1872-7905
• DOI: 10.1016/j.jim.2025.113921
• COBISS.SI-ID: 247676163
• Datum objave v RUL: 06.11.2025

Gradivo je del revije

• Naslov: Journal of immunological methods
• Založnik: Elsevier
• ISSN: 1872-7905
• COBISS.SI-ID: 26129113

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: dostavni sistemi, alergeni, mimotopi, Lactococcus lactis