20.500.12556/RUL-175626 Lactococcus lactis as a delivery system for surface displayed mimotopes of major peanut allergen Ara h 2 The prevalence of peanut allergy has been steadily increasing over the last decades. Among food allergies it shows the highest severity and is potentially fatal. Allergen Ara h 2 stands out as the main elicitor of allergic reactions. Research in the field of peanut immunotherapy still strives to improve the significant drawbacks of currently approved therapeutics. In this study we tested three epitope-like peptides as epitope-specific treatment. The epitope-like peptides (mimotopes) of major peanut allergen Ara h 2 were expressed as fusions with scaffold protein – lactococcal peptidoglycan binding domain of AcmA. Mimotope-decorated L. lactis and mimotope-AcmA fusion proteins were characterized. The mimotopes retained the ability to bind IgE from peanut-allergic patients' sera. They showed low basophil activation in peanut-allergic patients, except for mimotope DHPRYGP fused to AcmA suggesting its allergenic activity was partially preserved due to the highest similarity with Ara h 2 epitope. Mimotopes fused to AcmA can elicit T cell response; the increased secretion of IFN-γ suggests the shift of T cell immune response to non-allergic type 1. The use of recombinant L. lactis as a delivery system for surface displayed mimotopes represents a promising strategy that would enable delivery and expression of mimotopes on mucosal surfaces and at the same time beneficial immunomodulating properties. peanut allergy Ara h 2 Lactococcus lactis mimotopes dostavni sistemi alergeni mimotopi Lactococcus lactis true false true Angleški jezik Slovenski jezik Članek v reviji 2025-11-06 11:15:29 2025-11-06 11:15:33 2026-06-17 16:06:15 0000-00-00 00:00:00 2025 0 0 8 str. art. 113921 Vol. 543 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.015.14:616-056.43 1872-7905 10.1016/j.jim.2025.113921 247676163 1-s2.0-S0022175925001218-main.pdf 1-s2.0-S0022175925001218-main.pdf 1 163E4CE0313A464645819AA159276AD5 6d7e11c0473ccd318e9362d660ae33fcff384aebc06736e5cfce0e29d0486901 a2f74d81-bafa-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221457 https://www.sciencedirect.com/science/article/pii/S0022175925001218 1 36b21dad-baf9-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221456 Fakulteta za farmacijo 0 0 0