Phenotypic and genotypic antimicrobial resistance profiles of Flavobacterium psychrophilum and Flavobacterium branchiophilum isolated from rainbow trout (Oncorhynchus mykiss) in Slovenia

Pavlin, Katarina (avtor), Papić, Bojan (avtor), Zdovc, Irena (avtor), Knific, Tanja (avtor), Gruntar, Igor (avtor), Sitar, Rosvita (avtor), Žele-Vengušt, Diana (avtor), Seničar, Marija (avtor), Ocepek, Matjaž (avtor), Švara, Tanja (avtor)

Flavobacterium psychrophilum and Flavobacterium branchiophilum are important fish pathogens that cause considerable economic losses in freshwater aquaculture worldwide. Their antimicrobial resistance (AMR) profiles were evaluated using the latest Clinical and Laboratory Standards Institute broth microdilution method, which was adjusted for F. branchiophilum to achieve growth, and whole-genome sequencing (WGS). A total of 51 F. psychrophilum isolates and eight F. branchiophilum isolates from Slovenian farmed rainbow trout underwent phenotypic antimicrobial susceptibility testing. Overall, 86.3% of F. psychrophilum isolates were classified as non-wild-type (NWT) for oxytetracycline and enrofloxacin, and 90.2% of isolates were NWT for oxolinic acid. In contrast, all isolates tested were classified as wild-type (WT) for florfenicol and erythromycin. It was not possible to classify F. branchiophilum isolates as WT or NWT for the antimicrobials tested. A subset of F. psychrophilum (n = 7) and F. branchiophilum (n = 2) isolates was further characterised using WGS to investigate the mechanisms mediating antimicrobial resistance. In F. psychrophilum, the T83A or T83V substitution in GyrA was associated with reduced susceptibility to oxolinic acid. No other AMR genes or AMR-associated mutations were detected.

• Jezik: Angleški jezik
• Ključne besede: AMR profile, Flavobacterium branchiophilum, Flavobacterium psychrophilum, minimum inhibitory concentration, MIC, rainbow trout, Oncorhynchus mykiss, whole-genome sequencing, WGS
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 12 str.
• Številčenje: Vol. 48, iss. 7, art. e14119
• PID: 20.500.12556/RUL-174756
• UDK: 636.09:616
• ISSN pri članku: 1365-2761
• DOI: 10.1111/jfd.14119
• COBISS.SI-ID: 230430979
• Datum objave v RUL: 20.11.2025

Gradivo je del revije

• Naslov: Journal of fish diseases
• Skrajšan naslov: J. fish dis.
• Založnik: Wiley
• ISSN: 1365-2761
• COBISS.SI-ID: 515027481

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Program financ.: Young researchers

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana