20.500.12556/RUL-174756 Phenotypic and genotypic antimicrobial resistance profiles of Flavobacterium psychrophilum and Flavobacterium branchiophilum isolated from rainbow trout (Oncorhynchus mykiss) in Slovenia Flavobacterium psychrophilum and Flavobacterium branchiophilum are important fish pathogens that cause considerable economic losses in freshwater aquaculture worldwide. Their antimicrobial resistance (AMR) profiles were evaluated using the latest Clinical and Laboratory Standards Institute broth microdilution method, which was adjusted for F. branchiophilum to achieve growth, and whole-genome sequencing (WGS). A total of 51 F. psychrophilum isolates and eight F. branchiophilum isolates from Slovenian farmed rainbow trout underwent phenotypic antimicrobial susceptibility testing. Overall, 86.3% of F. psychrophilum isolates were classified as non-wild-type (NWT) for oxytetracycline and enrofloxacin, and 90.2% of isolates were NWT for oxolinic acid. In contrast, all isolates tested were classified as wild-type (WT) for florfenicol and erythromycin. It was not possible to classify F. branchiophilum isolates as WT or NWT for the antimicrobials tested. A subset of F. psychrophilum (n = 7) and F. branchiophilum (n = 2) isolates was further characterised using WGS to investigate the mechanisms mediating antimicrobial resistance. In F. psychrophilum, the T83A or T83V substitution in GyrA was associated with reduced susceptibility to oxolinic acid. No other AMR genes or AMR-associated mutations were detected. AMR profile Flavobacterium branchiophilum Flavobacterium psychrophilum minimum inhibitory concentration MIC rainbow trout Oncorhynchus mykiss whole-genome sequencing WGS true false true Angleški jezik Ni določen Članek v reviji 2025-10-10 04:41:28 2025-11-20 13:14:28 2025-12-11 04:39:41 0000-00-00 00:00:00 2025 0 0 12 str. iss. 7, art. e14119 Vol. 48 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616 1365-2761 10.1111/jfd.14119 230430979 Journal_of_Fish_Diseases_-_2025_-_Pavlin_-_Phenotypic_and_Genotypic_Antimicrobial_Resistance_Profiles_of_Flavobacterium.pdf Journal_of_Fish_Diseases_-_2025_-_Pavlin_-_Phenotypic_and_Genotypic_Antimicrobial_Resistance_Profiles_of_Flavobacterium.pdf 1 84C91AD94FD2289828F508687BB9A94E 997db3f1a84745dd8f6adf6763ba0fffd4684c47cc46c080d10c74d6d2e4e5f1 ef42894c-d5a0-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=223249 https://onlinelibrary.wiley.com/doi/10.1111/jfd.14119 1 66949c72-a582-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=219644 Veterinarska fakulteta 0 0 0