Antibiotic resistance is one of the greatest challenges of modern medicine and public health, as it reduces the effectiveness of treating infectious diseases. Since human, animal, and environmental health are directly linked (the One Health concept), interference with one of these elements poses a serious threat to the entire global ecosystem. Understanding the mechanisms of the emergence and spread of resistance, as well as the importance of the responsible use of antibiotics, must therefore be incorporated into the educational process. In this master’s thesis, we developed a didactic kit for conducting the laboratory exercise Bacterial Resistance to Antibiotics in general secondary school biology classes, with included all the necessary equipment and instructions. A list of all required materials and easily accessible alternatives is available, in case certain items are not available in the school. Two versions of the exercise (hands-on and minds-on) have been prepared, accompanied by a lesson plan for teachers and instructions for preparation. For students, theoretical background material has been designed as pre-exercise preparation, along with a worksheet containing instructions, space for recording observations, and discussion questions. The purpose of the kit is to familiarize students with the concepts of antibiotic resistance, microbiological techniques, and genetic foundations, while also developing research-oriented thinking and practical skills. The result of the thesis is a comprehensive teaching resource that enables the implementation of a more demanding laboratory exercise in the secondary school, where such content is rarely included in lessons due to technical and financial constraints. The prepared exercise provides students with active engagement in the issue of antibiotic resistance and its broader impact on society, thereby contributing to the development of scientific literacy and a responsible attitude toward the use of antibiotics.
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