A pipeline for the isolation and cultivation of microalgae and cyanobacteria from hypersaline environments

Tavčar Verdev, Petra (avtor), Dolinar, Marko (avtor)

Microorganisms in high-salinity environments play a critical role in biogeochemical cycles, primary production, and the biotechnological exploitation of extremozymes and bioactive compounds. The main challenges in current research include isolating and cultivating these microorganisms under laboratory conditions and understanding their complex adaptive mechanisms to high salinity. Currently, universally recognized protocols for isolating microalgae and cyanobacteria from salt pans, salterns, and similar natural habitats are lacking. Establishing axenic laboratory cultures is essential for identifying new species thriving in high-salinity environments and for exploring the synthesis of high-value metabolites by these microorganisms ex situ. Our ongoing research primarily focuses on photosynthetic microorganisms with significant biotechnological potential, particularly for skincare applications. By integrating data from the existing literature with our empirical findings, we propose a standardized pipeline for the isolation and laboratory cultivation of microalgae and cyanobacteria originating from aqueous environments characterized by elevated salt concentrations, such as solar salterns. This approach will be particularly useful for researchers working with microorganisms adapted to hypersaline waters.

• Jezik: Angleški jezik
• Ključne besede: salterns, salt pans, halophilic microorganisms, culture systems, axenic culture, biotechnology, Chlorophyta, Cyanoprokaryota
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 21 str.
• Številčenje: Vol. 13, iss. 3, art. 603
• PID: 20.500.12556/RUL-170834
• UDK: 582.232
• ISSN pri članku: 2076-2607
• DOI: 10.3390/microorganisms13030603
• COBISS.SI-ID: 229089027
• Datum objave v RUL: 17.07.2025

Gradivo je del revije

• Naslov: Microorganisms
• Skrajšan naslov: Microorganisms
• Založnik: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: soline, slanišča, halofilni mikroorganizmi, sistemi gojenja, biotehnologija, Chlorophyta, Cyanoprokaryota

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P1-0048-2018
• Naslov: Strukturna biologija

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: L4-4564-2022
• Naslov: Biotehnološki potencial izbranih mikroorganizmov iz Sečoveljskih solin za uporabo v kozmetičnih izdelkih