20.500.12556/RUL-170834 A pipeline for the isolation and cultivation of microalgae and cyanobacteria from hypersaline environments Microorganisms in high-salinity environments play a critical role in biogeochemical cycles, primary production, and the biotechnological exploitation of extremozymes and bioactive compounds. The main challenges in current research include isolating and cultivating these microorganisms under laboratory conditions and understanding their complex adaptive mechanisms to high salinity. Currently, universally recognized protocols for isolating microalgae and cyanobacteria from salt pans, salterns, and similar natural habitats are lacking. Establishing axenic laboratory cultures is essential for identifying new species thriving in high-salinity environments and for exploring the synthesis of high-value metabolites by these microorganisms ex situ. Our ongoing research primarily focuses on photosynthetic microorganisms with significant biotechnological potential, particularly for skincare applications. By integrating data from the existing literature with our empirical findings, we propose a standardized pipeline for the isolation and laboratory cultivation of microalgae and cyanobacteria originating from aqueous environments characterized by elevated salt concentrations, such as solar salterns. This approach will be particularly useful for researchers working with microorganisms adapted to hypersaline waters. salterns salt pans halophilic microorganisms culture systems axenic culture biotechnology Chlorophyta Cyanoprokaryota soline slanišča halofilni mikroorganizmi sistemi gojenja biotehnologija Chlorophyta Cyanoprokaryota true false true Angleški jezik Slovenski jezik Članek v reviji 2025-07-17 12:52:50 2025-07-17 12:52:51 2025-07-20 03:42:16 0000-00-00 00:00:00 2025 0 0 21 str. iss. 3, art. 603 Vol. 13 Mar. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 582.232 2076-2607 10.3390/microorganisms13030603 229089027 RAZ_Tavcar_Verdev_Petra_2025.pdf RAZ_Tavcar_Verdev_Petra_2025.pdf 1 F1BA6FCDCB406F22D0A62683A1805100 7473ab62e35194e414962f9d882c6590e0d5df5e642c0eec3ef02ae514560c67 a1e507db-62fa-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=213090 https://www.mdpi.com/2076-2607/13/3/603 1 3c2d5a08-62fa-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=213089 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0