Exposure to specific fungal lectins during adhesion impairs biofilm formation of Listeria on polystyrene

Janež, Nikolaja (avtor), Ladányi, Márta (avtor), Sterniša, Meta (avtor), Jug, Blaž (avtor), Zupan, Tanja (avtor), Peternel, Tjaša (avtor), Sebastijanović, Aleksandar (avtor), Perišić Nanut, Milica (avtor), Karničar, Katarina (avtor), Taler-Verčič, Ajda (avtor), Turk, Dušan (avtor), Klančnik, Anja (avtor), Štrancar, Janez (avtor), Sabotič, Jerica (avtor)

Listeria monocytogenes is a pathogenic bacterium that can form biofilms in food processing plants, allowing the bacteria to survive despite the control measures applied. As the surface of the bacteria is covered with versatile polysaccharides and proteins, these influence the interactions of the bacterium with any surface. The unique properties and high stability of fungal proteins make them good candidates for the control of bacteria by targeting surface structures. We screened a group of fungal lectins and protease inhibitors from different fungal species, protein folds and known targets for their antibacterial and antibiofilm activity against model strains of Listeria innocua and Listeria monocytogenes. Several of them significantly decreased the viability of biofilm bacteria, but had no effect on bacterial growth parameters at 37°C and thus had no antibacterial activity. Fungal lectins significantly impaired biofilm development even at room temperature, which was attributed to exposure to lectins during adhesion. The tested fungal proteins also reduced biofilm development on biological model surfaces. The observed antibiofilm activity of fungal proteins suggests that they have the potential to modulate interactions between bacteria and/or between bacteria and surfaces, which could be used in the future to reduce surface contamination by Listeria.

• Jezik: Angleški jezik
• Ključne besede: Listeria monocytogenes, biofilm
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 13 str.
• Številčenje: Vol. 17, iss. 12, art. e70040
• PID: 20.500.12556/RUL-169768
• UDK: 577
• ISSN pri članku: 1751-7915
• DOI: 10.1111/1751-7915.70040
• COBISS.SI-ID: 217485315
• Datum objave v RUL: 09.06.2025

Gradivo je del revije

• Naslov: Microbial biotechnology
• Skrajšan naslov: Microb. biotechnol.
• Založnik: Wiley
• ISSN: 1751-7915
• COBISS.SI-ID: 14597398

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Listeria monocytogenes, biofilm, polistiren, kontaminacija, listerijski meningitis