20.500.12556/RUL-169768 Exposure to specific fungal lectins during adhesion impairs biofilm formation of Listeria on polystyrene Listeria monocytogenes is a pathogenic bacterium that can form biofilms in food processing plants, allowing the bacteria to survive despite the control measures applied. As the surface of the bacteria is covered with versatile polysaccharides and proteins, these influence the interactions of the bacterium with any surface. The unique properties and high stability of fungal proteins make them good candidates for the control of bacteria by targeting surface structures. We screened a group of fungal lectins and protease inhibitors from different fungal species, protein folds and known targets for their antibacterial and antibiofilm activity against model strains of Listeria innocua and Listeria monocytogenes. Several of them significantly decreased the viability of biofilm bacteria, but had no effect on bacterial growth parameters at 37°C and thus had no antibacterial activity. Fungal lectins significantly impaired biofilm development even at room temperature, which was attributed to exposure to lectins during adhesion. The tested fungal proteins also reduced biofilm development on biological model surfaces. The observed antibiofilm activity of fungal proteins suggests that they have the potential to modulate interactions between bacteria and/or between bacteria and surfaces, which could be used in the future to reduce surface contamination by Listeria. Listeria monocytogenes biofilm Listeria monocytogenes biofilm polistiren kontaminacija listerijski meningitis true false true Angleški jezik Slovenski jezik Članek v reviji 2025-06-09 15:53:01 2025-06-09 15:53:07 2025-06-11 04:28:58 0000-00-00 00:00:00 2024 0 0 13 str. iss. 12, art. e70040 Vol. 17 Dec. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 577 1751-7915 10.1111/1751-7915.70040 217485315 Microbial_Biotechnology_-_2024_-_Janez_-_Exposure_to_specific_fungal_lectins_during_adhesion_impairs_biofilm_formation_of.pdf Microbial_Biotechnology_-_2024_-_Janez_-_Exposure_to_specific_fungal_lectins_during_adhesion_impairs_biofilm_formation_of.pdf 1 CF2A4482AB31058DB05B594837A80CF8 7d075d363b1633b55cf20e6de8e32b06659c7ad284ef7fe48ec5db29f7bd7b6e 7d96c327-4538-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211459 https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.70040 1 97d0753a-4537-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211458 Biotehniška fakulteta 0 0 0