Genetic characterization of Slovenian pear germplasm by microsatellite markers and flow cytometry

Gostinčar, Kristina (avtor), Osterc, Gregor (avtor), Štajner, Nataša (avtor), Luthar, Zlata (avtor)

In this work, a total of 132 genetic resources from four distinct collections in Slovenia were analysed with a set of 14 microsatellite markers (SSR) to determine the genetic structure and diversity of the pear germplasm. In total, 242 distinct alleles were successfully amplified with an average of 17.3 alleles per locus and the results showed a high diversity of the germplasm with an average expected heterozygosity (He) of 0.82 and an average observed heterozygosity (Ho) of 0.68. Pairwise comparison reduced the number of unique genetic resources to 108 (19 % redundancy). The study also revealed 25 genetic resources with synonymy and 15 cases of homonyms. The cluster analysis of unique genetic resources and 7 reference varieties grouped the accessions into three main groups. The group A and C consisted mostly of a local genotype, while the group B included in majority international varieties. In addition, group A includes also P. pyraster and P. caucasica. Using a Bayesian clustering method revealed three main genetic groups, G1 and G2 with predominantly local genotypes, G3 with international varieties. Flow cytometry confirmed the presence of 10 triploid genetic resources in the population studied, representing 9.7 %. Phenotypic evaluation revealed significant differences between the early and late harvest groups for fruit height, width and weight, while there were no differences for stalk length and thickness. The results indicate a high genetic diversity of the local germplasm and underline the importance of preserving these varieties, which may be required for new breeding programmes.

• Jezik: Angleški jezik
• Ključne besede: Pyrus spp., pear, genetic resources, genetic diversity, SSR, flow cytometry, ploidy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 9 str.
• Številčenje: Vol. 345, [article no.] ǂ114091
• PID: 20.500.12556/RUL-169126
• UDK: 575
• ISSN pri članku: 0304-4238
• DOI: 10.1016/j.scienta.2025.114091
• COBISS.SI-ID: 235714563
• Datum objave v RUL: 13.05.2025

Gradivo je del revije

• Naslov: Scientia horticulturae
• Skrajšan naslov: Sci. hortic.
• Založnik: Elsevier
• ISSN: 0304-4238
• COBISS.SI-ID: 5191687

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: hruška, genski viri, genetska raznolikost, mikrosatelitski markerji, pretočna citometrija, ploidnost

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Naslov: Young Researcher program

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0077
• Naslov: Kmetijske rastline - genetika in sodobne tehnologije