20.500.12556/RUL-169126 Genetic characterization of Slovenian pear germplasm by microsatellite markers and flow cytometry In this work, a total of 132 genetic resources from four distinct collections in Slovenia were analysed with a set of 14 microsatellite markers (SSR) to determine the genetic structure and diversity of the pear germplasm. In total, 242 distinct alleles were successfully amplified with an average of 17.3 alleles per locus and the results showed a high diversity of the germplasm with an average expected heterozygosity (He) of 0.82 and an average observed heterozygosity (Ho) of 0.68. Pairwise comparison reduced the number of unique genetic resources to 108 (19 % redundancy). The study also revealed 25 genetic resources with synonymy and 15 cases of homonyms. The cluster analysis of unique genetic resources and 7 reference varieties grouped the accessions into three main groups. The group A and C consisted mostly of a local genotype, while the group B included in majority international varieties. In addition, group A includes also P. pyraster and P. caucasica. Using a Bayesian clustering method revealed three main genetic groups, G1 and G2 with predominantly local genotypes, G3 with international varieties. Flow cytometry confirmed the presence of 10 triploid genetic resources in the population studied, representing 9.7 %. Phenotypic evaluation revealed significant differences between the early and late harvest groups for fruit height, width and weight, while there were no differences for stalk length and thickness. The results indicate a high genetic diversity of the local germplasm and underline the importance of preserving these varieties, which may be required for new breeding programmes. Pyrus spp. pear genetic resources genetic diversity SSR flow cytometry ploidy hruška genski viri genetska raznolikost mikrosatelitski markerji pretočna citometrija ploidnost true false true Angleški jezik Slovenski jezik Članek v reviji 2025-05-13 13:18:47 2025-05-13 13:18:53 2026-06-18 04:33:50 0000-00-00 00:00:00 2025 0 0 9 str. art. 114091 Vol. 345 Apr. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 575 0304-4238 10.1016/j.scienta.2025.114091 235714563 1-s2.0-S0304423825001426-main.pdf 1-s2.0-S0304423825001426-main.pdf 1 EB69EE3CC3F60DF52034C7E3DDCA1EB7 41973feda65ba74b9e05d7f19d0813a818d52c50f4d17d5e30c5d9b9dca1f468 d6c49b09-6a27-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235830 https://www.sciencedirect.com/science/article/pii/S0304423825001426 1 d67788e9-2fea-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210304 Biotehniška fakulteta 0 0 0