Monitoring electroporation-induced changes in action potential generation in genetically engineered tet-on spiking HEK cells

Batista Napotnik, Tina (avtor), Kos, Bor (avtor), Rems, Lea (avtor)

Excitable cells such as neuronal and muscle cells can be primary targets in rapidly emerging electroporation-based treatments. However, they can be affected by electric pulses even in therapies where they are not the primary targets, and this can cause adverse side effects. Therefore, to optimize the electroporation-based treatments of excitable and non-excitable tissues, there is a need to study the effects of electric pulses on excitable cells, their ion channels, and excitability in vitro. For this purpose, a protocol was developed for optical monitoring of changes in action potential generation due to electroporation on a simple excitable cell model of genetically engineered tet-on spiking HEK cells. With the use of a fluorescent potentiometric dye, the changes in transmembrane voltage were monitored under a fluorescence microscope, and relevant parameters of cell responses were extracted automatically with a MATLAB application. This way, the excitable cell responses to different electric pulses and the interplay between excitation and electroporation could be efficiently evaluated.

• Jezik: Angleški jezik
• Ključne besede: electroporation, excitable cells, action potential
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Recenzirani rokopis
• Leto izida: 2024
• Št. strani: 12 str.
• Številčenje: Vol. 211, art. e67257
• PID: 20.500.12556/RUL-168459
• UDK: 602.621
• ISSN pri članku: 1940-087X
• DOI: 10.3791/67257
• COBISS.SI-ID: 230333955
• Datum objave v RUL: 14.04.2025

Gradivo je del revije

• Naslov: Journal of visualized experiments
• Skrajšan naslov: J. vis. exp.
• Založnik: MYJoVE Corporation
• ISSN: 1940-087X
• COBISS.SI-ID: 6867284

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: elektroporacija, vzdražne celice, akcijski potencial