20.500.12556/RUL-168459 Monitoring electroporation-induced changes in action potential generation in genetically engineered tet-on spiking HEK cells Excitable cells such as neuronal and muscle cells can be primary targets in rapidly emerging electroporation-based treatments. However, they can be affected by electric pulses even in therapies where they are not the primary targets, and this can cause adverse side effects. Therefore, to optimize the electroporation-based treatments of excitable and non-excitable tissues, there is a need to study the effects of electric pulses on excitable cells, their ion channels, and excitability in vitro. For this purpose, a protocol was developed for optical monitoring of changes in action potential generation due to electroporation on a simple excitable cell model of genetically engineered tet-on spiking HEK cells. With the use of a fluorescent potentiometric dye, the changes in transmembrane voltage were monitored under a fluorescence microscope, and relevant parameters of cell responses were extracted automatically with a MATLAB application. This way, the excitable cell responses to different electric pulses and the interplay between excitation and electroporation could be efficiently evaluated. electroporation excitable cells action potential elektroporacija vzdražne celice akcijski potencial true false true Angleški jezik Slovenski jezik Članek v reviji 2025-04-14 13:18:02 2025-04-14 13:18:08 2025-06-19 10:31:53 0000-00-00 00:00:00 2024 0 0 12 str. art. e67257 Vol. 211 2024 0000-00-00 PostprintKoncna Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 602.621 1940-087X 10.3791/67257 230333955 67257_R0_-_reviewed_R1_TBN_changes_acc.pdf 67257_R0_-_reviewed_R1_TBN_changes_acc.pdf 1 5B9AB6D2BD2D287D8936112AD166F415 f665a0f8e80d3d1de10157ac523d257f0a6ba1540f618983ed12b92af2ad492c cf4c61bd-19d8-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201985 https://app.jove.com/t/67257/monitoring-electroporation-induced-changes-action-potential 1 08ee7b2a-1921-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=201954 Fakulteta za elektrotehniko 0 0 0