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The α- to γ-enolase switch : the role and regulation of γ-enolase during oligodendrocyte differentiation
ID Horvat, Selena (Avtor), ID Pečar Fonović, Urša (Avtor), ID Mitrović, Ana (Avtor), ID Zidar, Nace (Avtor), ID Kos, Janko (Avtor), ID Pišlar, Anja (Avtor)

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Izvleček
The glycolytic enzyme γ-enolase is a highly specific neuronal marker that is known to replace ubiquitously expressed α-enolase in the brain. Moreover, γ-enolase has been shown to exert neurotrophic activity, which is regulated by cathepsin X, a lysosomal peptidase. This study investigates the role of γ-enolase and its regulation by cathepsin X during the differentiation of oligodendrocytes, which are essential for normal brain function. We established a differentiation protocol for the human oligodendroglioma (HOG) cell line and demonstrated for the first time that an α- to γ-enolase switch occurs during HOG cell differentiation. This switch was confirmed by the expression of specific markers underscoring the role of γ-enolase in oligodendrocyte differentiation. Moreover, γ-enolase overexpression enhanced oligodendrocyte differentiation, while silencing of γ-enolase by siRNA significantly decreased maturation marker. Further, the regulatory role of cysteine peptidase cathepsin X on γ-enolase function was found. Silencing cathepsin X significantly changed cell morphology, enhanced oligodendrocyte differentiation, altered the expression of oligodendrocyte markers, and increased levels of the active form of γ-enolase. Inhibiting cathepsin X similarly changed cell morphology and enhanced oligodendrocyte differentiation. These findings suggest that cathepsin X modulates γ-enolase activity and thereby influences oligodendrocyte differentiation and thus neuronal function.

Jezik:Angleški jezik
Ključne besede:oligodendrocytes, differentiation, γ-enolase, α-enolase, cathepsin X
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2025
Št. strani:15 str.
Številčenje:Vol. 301, art. 140464
PID:20.500.12556/RUL-167011 Povezava se odpre v novem oknu
UDK:579
ISSN pri članku:1879-0003
DOI:10.1016/j.ijbiomac.2025.140464 Povezava se odpre v novem oknu
COBISS.SI-ID:224856579 Povezava se odpre v novem oknu
Datum objave v RUL:03.02.2025
Število ogledov:333
Število prenosov:38
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:International journal of biological macromolecules
Skrajšan naslov:Int. j. biol. macromol.
Založnik:Elsevier
ISSN:1879-0003
COBISS.SI-ID:107163139 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:oligodendrociti, diferenciacija, γ-enolaza, α-enolaza, katepsin X

Projekti

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0127
Naslov:Farmacevtska biotehnologija: znanost za zdravje

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