The α- to γ-enolase switch : the role and regulation of γ-enolase during oligodendrocyte differentiation

Horvat, Selena (avtor), Pečar Fonović, Urša (avtor), Mitrović, Ana (avtor), Zidar, Nace (avtor), Kos, Janko (avtor), Pišlar, Anja (avtor)

The glycolytic enzyme γ-enolase is a highly specific neuronal marker that is known to replace ubiquitously expressed α-enolase in the brain. Moreover, γ-enolase has been shown to exert neurotrophic activity, which is regulated by cathepsin X, a lysosomal peptidase. This study investigates the role of γ-enolase and its regulation by cathepsin X during the differentiation of oligodendrocytes, which are essential for normal brain function. We established a differentiation protocol for the human oligodendroglioma (HOG) cell line and demonstrated for the first time that an α- to γ-enolase switch occurs during HOG cell differentiation. This switch was confirmed by the expression of specific markers underscoring the role of γ-enolase in oligodendrocyte differentiation. Moreover, γ-enolase overexpression enhanced oligodendrocyte differentiation, while silencing of γ-enolase by siRNA significantly decreased maturation marker. Further, the regulatory role of cysteine peptidase cathepsin X on γ-enolase function was found. Silencing cathepsin X significantly changed cell morphology, enhanced oligodendrocyte differentiation, altered the expression of oligodendrocyte markers, and increased levels of the active form of γ-enolase. Inhibiting cathepsin X similarly changed cell morphology and enhanced oligodendrocyte differentiation. These findings suggest that cathepsin X modulates γ-enolase activity and thereby influences oligodendrocyte differentiation and thus neuronal function.

• Jezik: Angleški jezik
• Ključne besede: oligodendrocytes, differentiation, γ-enolase, α-enolase, cathepsin X
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 15 str.
• Številčenje: Vol. 301, art. 140464
• PID: 20.500.12556/RUL-167011
• UDK: 579
• ISSN pri članku: 1879-0003
• DOI: 10.1016/j.ijbiomac.2025.140464
• COBISS.SI-ID: 224856579
• Datum objave v RUL: 03.02.2025

HORVAT, Selena, PEČAR FONOVIĆ, Urša, MITROVIĆ, Ana, ZIDAR, Nace, KOS, Janko in PIŠLAR, Anja, 2025, The α- to γ-enolase switch : the role and regulation of γ-enolase during oligodendrocyte differentiation. International journal of biological macromolecules [na spletu]. 2025. Vol. 301, no. 140464. [Dostopano 16 marec 2025]. DOI 10.1016/j.ijbiomac.2025.140464. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=167011

Gradivo je del revije

• Naslov: International journal of biological macromolecules
• Skrajšan naslov: Int. j. biol. macromol.
• Založnik: Elsevier
• ISSN: 1879-0003
• COBISS.SI-ID: 107163139

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: oligodendrociti, diferenciacija, γ-enolaza, α-enolaza, katepsin X

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0127
• Naslov: Farmacevtska biotehnologija: znanost za zdravje