Comprehensive analysis of 66 complete molluscum contagiosum virus (MOCV) genomes : characterization and functional annotation of 47 novel complete MOCV genomes, including the first genome of MOCV genotype 3, and a proposal for harmonized MOCV genotyping indexing

Zorec, Tomaž Mark (avtor), Alm, Erik (avtor), Lind Karlberg, Maria (avtor), Advani, Reza (avtor), Hošnjak, Lea (avtor), Poljak, Mario (avtor)

Four molluscum contagiosum virus (MOCV) genotypes (MOCV1–4) and four subtype variants (MOCV1p, MOCV1va, MOCV1vb, and MOCV1vc) were partially characterized using restriction enzyme profiling in the early 1980s/1990s. However, complete genome sequences of only MOCV1 and MOCV2 are available. The evolutionary pathways of MOCV genotypes and subtype variants with unavailable sequences remain unclear, and also whether all MOCV genotypes/subtype variants can be reliably detected and appropriately categorized using available PCR-based protocols. We de novo fully characterized and functionally annotated 47 complete MOCV genomes, including two putative non-MOCV1/2 isolates, expanding the number of fully characterized MOCV genomes to 66. To ascertain the placement of any putative novel MOCV sequence into the restriction profiling typing scheme, we developed an original framework for extracting complete MOCV genome sequence-based restriction profiles and matching them with reference restriction profiles. We confirmed that two putative non-MOCV1/2 isolates represent the first complete genomes of MOCV3. Comprehensive phylogenomic, recombination, and restriction enzyme recognition site analysis of all 66 currently available MOCV genomes showed that they can be agglomerated into six phylogenetic subgroups (PG1–6), corresponding to the subtype variants from the pioneering studies. PG5 was a novel subtype variant of MOCV2, but no PGs corresponded to the subtype variants MOCV1vb or MOCV4. We showed that the phylogenetic subgroups may have diverged from the prototype MOCV genotype lineages following large-scale recombination events and hinted at partial sequence content of MOCV4 and direction of recombinant transfer in the events that spawned PG5 and the yet undetected subtype variant MOCV1vb.

• Jezik: Angleški jezik
• Ključne besede: complete genome restriction mapping, complete genome sequencing, de novo assembly, genome annotation, genomic diversity, molluscipoxvirus harmonization, molluscum contagiosum virus, molluscum contagiosum virus genotyping, recombination
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 18 str.
• Številčenje: Vol. 14, iss. 6, art. e02224-23
• PID: 20.500.12556/RUL-164649
• UDK: 616-097:579
• ISSN pri članku: 2150-7511
• DOI: 10.1128/mbio.02224-23
• COBISS.SI-ID: 172346371
• Datum objave v RUL: 06.11.2024

Gradivo je del revije

• Naslov: mBio
• Skrajšan naslov: mBio
• Založnik: American Society for Microbiology
• ISSN: 2150-7511
• COBISS.SI-ID: 3503482

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: popolno kartiranje omejitve genoma, popolno sekvenciranje genoma, anotacija genoma, genomska raznolikost, harmonizacija moluscipoksvirusa, virus nalezljivega moluskuma, genotipizacija virusa nalezljivega moluskuma, rekombinacija

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0083
• Naslov: Odnosi parazitskega obstajanja

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: University of Ljubljana, Faculty of Medicine, Institute of Microbiology and Immunology