Comprehensive analysis of 66 complete molluscum contagiosum virus (MOCV) genomes : characterization and functional annotation of 47 novel complete MOCV genomes, including the first genome of MOCV genotype 3, and a proposal for harmonized MOCV genotyping indexing

Zorec, Tomaž Mark (author), Alm, Erik (author), Lind Karlberg, Maria (author), Advani, Reza (author), Hošnjak, Lea (author), Poljak, Mario (author)

Four molluscum contagiosum virus (MOCV) genotypes (MOCV1–4) and four subtype variants (MOCV1p, MOCV1va, MOCV1vb, and MOCV1vc) were partially characterized using restriction enzyme profiling in the early 1980s/1990s. However, complete genome sequences of only MOCV1 and MOCV2 are available. The evolutionary pathways of MOCV genotypes and subtype variants with unavailable sequences remain unclear, and also whether all MOCV genotypes/subtype variants can be reliably detected and appropriately categorized using available PCR-based protocols. We de novo fully characterized and functionally annotated 47 complete MOCV genomes, including two putative non-MOCV1/2 isolates, expanding the number of fully characterized MOCV genomes to 66. To ascertain the placement of any putative novel MOCV sequence into the restriction profiling typing scheme, we developed an original framework for extracting complete MOCV genome sequence-based restriction profiles and matching them with reference restriction profiles. We confirmed that two putative non-MOCV1/2 isolates represent the first complete genomes of MOCV3. Comprehensive phylogenomic, recombination, and restriction enzyme recognition site analysis of all 66 currently available MOCV genomes showed that they can be agglomerated into six phylogenetic subgroups (PG1–6), corresponding to the subtype variants from the pioneering studies. PG5 was a novel subtype variant of MOCV2, but no PGs corresponded to the subtype variants MOCV1vb or MOCV4. We showed that the phylogenetic subgroups may have diverged from the prototype MOCV genotype lineages following large-scale recombination events and hinted at partial sequence content of MOCV4 and direction of recombinant transfer in the events that spawned PG5 and the yet undetected subtype variant MOCV1vb.

• Language: English
• Keywords: complete genome restriction mapping, complete genome sequencing, de novo assembly, genome annotation, genomic diversity, molluscipoxvirus harmonization, molluscum contagiosum virus, molluscum contagiosum virus genotyping, recombination
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 18 str.
• Numbering: Vol. 14, iss. 6, art. e02224-23
• PID: 20.500.12556/RUL-164649
• UDC: 616-097:579
• ISSN on article: 2150-7511
• DOI: 10.1128/mbio.02224-23
• COBISS.SI-ID: 172346371
• Publication date in RUL: 06.11.2024

Record is a part of a journal

• Title: mBio
• Shortened title: mBio
• Publisher: American Society for Microbiology
• ISSN: 2150-7511
• COBISS.SI-ID: 3503482

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: popolno kartiranje omejitve genoma, popolno sekvenciranje genoma, anotacija genoma, genomska raznolikost, harmonizacija moluscipoksvirusa, virus nalezljivega moluskuma, genotipizacija virusa nalezljivega moluskuma, rekombinacija

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0083
• Name: Odnosi parazitskega obstajanja

• Funder: Other - Other funder or multiple funders
• Funding programme: University of Ljubljana, Faculty of Medicine, Institute of Microbiology and Immunology