Influence of archaeal lipids isolated from Aeropyrum pernix K1 on physicochemical properties of sphingomyelin-cholesterol liposomes

Kejžar, Jan (avtor), Mrak, Polona (avtor), Osojnik Črnivec, Ilja Gasan (avtor), Poklar Ulrih, Nataša (avtor)

We investigated the influence of archaeal lipids (C$_{25,25}$) isolated from thermophilic archaeon Aeropyrum pernix K1 on physicochemical properties of liposomes comprised of egg sphingomyelin (SM) and cholesterol (CH) using fluorescence emission anisotropy, calcein release studies, dynamic light scattering, transmission electron microscopy and phase analysis light scattering. The 2 mol% addition of archaeal lipids enabled formation of small unilamellar vesicles by sonication while also having significant effect on reducing mean size, polydispersity index and zeta potential of C$_{25,25}$/SM/CH vesicles. Increasing the ratio of C$_{25,25}$ lipids in mixture of C$_{25,25}$/SM/CH decreased lipid ordering parameter in dose dependent manner at different temperatures. We also demonstrated that adding 15 mol% C$_{25,25}$ to SM/CH mixture will cause it to notably interact with fetal bovine serum which could make them a viable alternative adjuvant to synthetic ether-linked lipids in development of advanced liposomal vaccine delivery systems. The prospect of combining the proven strengths of SM/CH mixtures with the unique properties of C$_{25,25}$ opens exciting possibilities for advancing drug delivery technologies, promising to yield formulations that are both highly effective and adaptable to a range of therapeutic applications.

• Jezik: Angleški jezik
• Ključne besede: liposomes, archaeal lipids, sphingomyelin, cholesterol, membrane fluidity, drug delivery
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 9 str.
• Številčenje: Vol. 1866, iss. 7, art. 184374
• PID: 20.500.12556/RUL-160546
• UDK: 577.35:582.233:615.014.6
• ISSN pri članku: 0005-2736
• DOI: 10.1016/j.bbamem.2024.184374
• COBISS.SI-ID: 205727235
• Datum objave v RUL: 30.08.2024

Gradivo je del revije

• Naslov: Biochimica et biophysica acta. Biomembranes
• Skrajšan naslov: Biochim. biophys. acta, Biomembr.
• Založnik: Elsevier
• ISSN: 0005-2736
• COBISS.SI-ID: 4077101

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: liposomi, lipidi arhej, sfingomielin, holesterol, fizikalnokemijske lastnosti, dostavni sistemi