20.500.12556/RUL-160546 Influence of archaeal lipids isolated from Aeropyrum pernix K1 on physicochemical properties of sphingomyelin-cholesterol liposomes We investigated the influence of archaeal lipids (C$_{25,25}$) isolated from thermophilic archaeon Aeropyrum pernix K1 on physicochemical properties of liposomes comprised of egg sphingomyelin (SM) and cholesterol (CH) using fluorescence emission anisotropy, calcein release studies, dynamic light scattering, transmission electron microscopy and phase analysis light scattering. The 2 mol% addition of archaeal lipids enabled formation of small unilamellar vesicles by sonication while also having significant effect on reducing mean size, polydispersity index and zeta potential of C$_{25,25}$/SM/CH vesicles. Increasing the ratio of C$_{25,25}$ lipids in mixture of C$_{25,25}$/SM/CH decreased lipid ordering parameter in dose dependent manner at different temperatures. We also demonstrated that adding 15 mol% C$_{25,25}$ to SM/CH mixture will cause it to notably interact with fetal bovine serum which could make them a viable alternative adjuvant to synthetic ether-linked lipids in development of advanced liposomal vaccine delivery systems. The prospect of combining the proven strengths of SM/CH mixtures with the unique properties of C$_{25,25}$ opens exciting possibilities for advancing drug delivery technologies, promising to yield formulations that are both highly effective and adaptable to a range of therapeutic applications. liposomes archaeal lipids sphingomyelin cholesterol membrane fluidity drug delivery liposomi lipidi arhej sfingomielin holesterol fizikalnokemijske lastnosti dostavni sistemi true false true Angleški jezik Slovenski jezik Članek v reviji 2024-08-30 13:50:49 2024-08-30 13:50:59 2024-09-01 03:27:56 0000-00-00 00:00:00 2024 0 0 9 str. iss. 7, art. 184374 Vol. 1866 Oct. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.35:582.233:615.014.6 0005-2736 10.1016/j.bbamem.2024.184374 205727235 1-s2.0-S0005273624001056-main.pdf 1-s2.0-S0005273624001056-main.pdf 1 18CCB02F2BBC131BAFE54D72C974487B e7e3e85bfa33526c7bd1c56e83d3bba1771f58be12f3ff4c22fbd73f22476c88 32c8c337-66c6-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=189276 https://www.sciencedirect.com/science/article/pii/S0005273624001056 0 e3eaf1dc-66c5-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=189275 Biotehniška fakulteta 0 0 0