Association of Mycoplasma canis with fertility disorders in dogs : a case study supported by clinical examination, PCR, 16S microbiota profiling, and serology

Suhadolc, Sara (avtor), Slavec, Brigita (avtor), Klinc, Primož (avtor), Tozon, Nataša (avtor), Papić, Bojan (avtor), Koprivec, Saša (avtor)

The role of Mycoplasma canis in canine fertility disorders is still poorly understood. As infection is often asymptomatic, there is an increasing need for appropriate diagnostic methods and treatment plans that would allow the reliable detection of M. canis infection and rapid alleviation of infection symptoms in affected dogs. In this study, we included 14 dogs with fertility problems and 16 dogs without fertility disorder signs. We compared clinical examination data and selected laboratory parameters (hematology and biochemistry) between the groups. We performed PCR-based detection of M. canis and 16S rRNA gene-based microbiota profiling of DNA isolated from vaginal and preputial swabs. Dog sera were tested for the presence of M. canis-specific antibodies. Hematological and selected biochemical parameters showed no differences between groups. PCR-based detection of M. canis in the samples was consistent with the results of 16S microbiota profiling. Several other bacterial taxa were also identified that could potentially be involved in different fertility disorders. Serological methods were not accurate enough since high cross-reactivity rates were observed. In the future, more accurate and efficient methods will be needed to determine the role of M. canis and its true role in the pathogenesis of specific fertility disorders in dogs.

• Jezik: Angleški jezik
• Ključne besede: mycoplasma, canine fertility disorders, vaginal swab, microbiota, specific antibodies
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 17 str.
• Številčenje: Vol. 13, iss. 5, art. 391
• PID: 20.500.12556/RUL-158611
• UDK: 579:636.7.09:616
• ISSN pri članku: 2076-0817
• DOI: 10.3390/pathogens13050391
• COBISS.SI-ID: 194880515
• Datum objave v RUL: 18.06.2024

Gradivo je del revije

• Naslov: Pathogens
• Skrajšan naslov: Pathogens
• Založnik: MDPI
• ISSN: 2076-0817
• COBISS.SI-ID: 523338009

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 16.06.2024

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikoplazma, motnje plodnosti psov, vaginalni bris, mikrobiota, specifična protitelesa

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0053
• Naslov: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana