20.500.12556/RUL-158611 Association of Mycoplasma canis with fertility disorders in dogs a case study supported by clinical examination, PCR, 16S microbiota profiling, and serology The role of Mycoplasma canis in canine fertility disorders is still poorly understood. As infection is often asymptomatic, there is an increasing need for appropriate diagnostic methods and treatment plans that would allow the reliable detection of M. canis infection and rapid alleviation of infection symptoms in affected dogs. In this study, we included 14 dogs with fertility problems and 16 dogs without fertility disorder signs. We compared clinical examination data and selected laboratory parameters (hematology and biochemistry) between the groups. We performed PCR-based detection of M. canis and 16S rRNA gene-based microbiota profiling of DNA isolated from vaginal and preputial swabs. Dog sera were tested for the presence of M. canis-specific antibodies. Hematological and selected biochemical parameters showed no differences between groups. PCR-based detection of M. canis in the samples was consistent with the results of 16S microbiota profiling. Several other bacterial taxa were also identified that could potentially be involved in different fertility disorders. Serological methods were not accurate enough since high cross-reactivity rates were observed. In the future, more accurate and efficient methods will be needed to determine the role of M. canis and its true role in the pathogenesis of specific fertility disorders in dogs. mycoplasma canine fertility disorders vaginal swab microbiota specific antibodies mikoplazma motnje plodnosti psov vaginalni bris mikrobiota specifična protitelesa true false true Angleški jezik Slovenski jezik Članek v reviji 2024-06-18 07:35:16 2024-06-18 07:35:20 2024-07-02 03:26:09 0000-00-00 00:00:00 2024 0 0 17 str. iss. 5, art. 391 Vol. 13 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579:636.7.09:616 2076-0817 10.3390/pathogens13050391 194880515 Association-Koprivec.pdf Association-Koprivec.pdf 1 90045CEEA7059975A1965E96B2E2B2EC 9c28ef602722391938f7c6658147291daf8fa739d3202ef6b014bf38e35d4593 49757a8b-2d35-11ef-88cb-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186297 https://www.mdpi.com/2076-0817/13/5/391 1 8e0d4f67-2d34-11ef-88cb-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186296 Veterinarska fakulteta 0 0 0