Study of V$_2$CT$_x$-MXene based immunosensor for sensitive label-free impedimetric detection of SARS-CoV-2 spike protein

Tasić, Nikola (avtor), Konjević, Ivan (avtor), Lobato, Alnilan Cristina Barros (avtor), Metarapi, Dino (avtor), Finšgar, Matjaž (avtor), Oliveira, Filipa M. (avtor), Sofer, Zdeněk (avtor), Gusmão, Rui (avtor), Zhang, Xueji (avtor), Hočevar, Samo B. (avtor)

Rapid and reliable immunosensing is undoubtedly one of the priorities in the efficient management and combat against a pandemic, as society has experienced with the SARS-CoV-2 outbreak; simple and cost-effective sensing strategies are at the forefront of these efforts. In this regard, 2D-layered MXenes hold great potential for electrochemical biosensing due to their attractive physicochemical properties. Herein, we present a V$_2$CT$_x$ MXene-based sensing layer as an integral part of a label-free immunosensor for sensitive and selective detection of the SARS-CoV-2 spike protein. The sensor was fabricated on a supporting screen-printed carbon electrode using Nafion as an immobilizing agent for MXene and glutaraldehyde, the latter enabling effective binding of protein A for further site-oriented immobilization of anti-SARS-CoV-2 antibodies. A thorough structural analysis of the sensor architecture was carried out, and several key parameters affecting the fabrication and analytical performance of the immunosensor were investigated and optimized. The immunosensor showed excellent electroanalytical performance in combination with an impedimetric approach and exhibited a low detection limit of only 45 fM SARS-CoV-2 spike protein. Its practical applicability was successfully demonstrated by measuring the spike protein in a spiked artificial nasopharyngeal fluid sample.

• Jezik: Angleški jezik
• Ključne besede: spike protein, immunosensors, SARS-CoV-2, V$_2$CT$_x$ MXene, SARS-CoV-2, electrochemical behavior, impedimetric detection
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 30196-30208
• Številčenje: Vol. 16, iss. 23
• PID: 20.500.12556/RUL-158476
• UDK: 543.384
• ISSN pri članku: 1944-8252
• DOI: 10.1021/acsami.4c04567
• COBISS.SI-ID: 198606083
• Datum objave v RUL: 13.06.2024

Gradivo je del revije

• Naslov: ACS applied materials & interfaces
• Skrajšan naslov: ACS appl. mater. interfaces
• Založnik: American Chemical Society
• ISSN: 1944-8252
• COBISS.SI-ID: 516049433

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: imunosenzorji, polprevodniške nanostrukture, proteini