20.500.12556/RUL-158476 Study of V$_2$CT$_x$-MXene based immunosensor for sensitive label-free impedimetric detection of SARS-CoV-2 spike protein Rapid and reliable immunosensing is undoubtedly one of the priorities in the efficient management and combat against a pandemic, as society has experienced with the SARS-CoV-2 outbreak; simple and cost-effective sensing strategies are at the forefront of these efforts. In this regard, 2D-layered MXenes hold great potential for electrochemical biosensing due to their attractive physicochemical properties. Herein, we present a V$_2$CT$_x$ MXene-based sensing layer as an integral part of a label-free immunosensor for sensitive and selective detection of the SARS-CoV-2 spike protein. The sensor was fabricated on a supporting screen-printed carbon electrode using Nafion as an immobilizing agent for MXene and glutaraldehyde, the latter enabling effective binding of protein A for further site-oriented immobilization of anti-SARS-CoV-2 antibodies. A thorough structural analysis of the sensor architecture was carried out, and several key parameters affecting the fabrication and analytical performance of the immunosensor were investigated and optimized. The immunosensor showed excellent electroanalytical performance in combination with an impedimetric approach and exhibited a low detection limit of only 45 fM SARS-CoV-2 spike protein. Its practical applicability was successfully demonstrated by measuring the spike protein in a spiked artificial nasopharyngeal fluid sample. spike protein immunosensors SARS-CoV-2 V$_2$CT$_x$ MXene SARS-CoV-2 electrochemical behavior impedimetric detection imunosenzorji polprevodniške nanostrukture proteini true false true Angleški jezik Slovenski jezik Članek v reviji 2024-06-13 16:11:37 2024-06-13 16:11:39 2024-06-29 03:26:18 0000-00-00 00:00:00 2024 0 0 Str. 30196-30208 iss. 23 Vol. 16 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.384 1944-8252 10.1021/acsami.4c04567 198606083 tasic-et-al-2024-study-of-v2ctx-mxene-based-immunosensor-for-sensitive-label-free-impedimetric-detection-of-sars-cov-2.pdf tasic-et-al-2024-study-of-v2ctx-mxene-based-immunosensor-for-sensitive-label-free-impedimetric-detection-of-sars-cov-2.pdf 1 EBCD296B4F2CB2179810C88E69302611 6609e7b1230d4e4f42ed9e28aae516a09bb29e28e8067ca2cb4449c9aa17a7d0 0ec391ff-298f-11ef-81c1-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186116 https://pubs.acs.org/doi/10.1021/acsami.4c04567 0 033f610a-298f-11ef-81c1-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186115 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0