Invasive alien species as a potential source of phytopharmaceuticals : Phenolic composition and antimicrobial and cytotoxic activity of Robinia pseudoacacia L. leaf and flower extracts

Uzelac, Mirela (avtor), Sladonja, Barbara (avtor), Šola, Ivana (avtor), Dudaš, Slavica (avtor), Mikulič Petkovšek, Maja (avtor), Poljuha, Danijela (avtor)

Black locust (Robinia pseudoacacia L.), an invasive tree in Europe, commonly known for its negative impact on biodiversity, is a rich source of phenolic compounds recognized in traditional medicine. Since the metabolite profile depends on the environment and climate, this study aimed to provide the first LC-MS phytochemical screening of the black locust from the Istria region (Croatia). The compounds were extracted from leaves and flowers with 70% ethanol and 80% methanol. Total phenolics (TP) and flavonoids (TF), as well as antioxidant capacity (AC) measured by ABTS (17.49–146.41 mg TE/g DW), DPPH (24.67–118.49 mg TE/g DW), and FRAP (7.38–77.53 mg TE/g DW) assays, were higher in leaf than in flower extracts. Higher TP and total non-flavonoid (TNF) values were displayed in ethanolic than in methanolic extracts. In total, 64 compounds were identified, of which flavonols (20) and hydroxycinnamic acid derivatives (15) were the most represented. Flavanols such as catechin dominated in leaf extracts, followed by flavonols, with kaempferol glucuronyl rhamnosyl hexosides as the main compound, respectively. Flower extracts had the highest share of flavones, followed by ellagitannins, with luteolin dirhamnosyl hexosides and vescalagin, respectively, being predominant. The extracts had good quorum sensing, biofilm formation prevention, and eradicating capacity. The results provided new insights into the phytochemical properties of R. pseudoacacia as the first step toward its potential pharmaceutical use.

• Jezik: Angleški jezik
• Ključne besede: antioxidant, antibacterial, antibiofilm, antifungal, anti-quorum sensing, black locust, phenolics
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 23 str.
• Številčenje: Vol. 12, iss. 14, art. 2715
• PID: 20.500.12556/RUL-153017
• UDK: 634.1/.7
• ISSN pri članku: 2223-7747
• COBISS.SI-ID: 177421571
• Datum objave v RUL: 14.12.2023

Gradivo je del revije

• Naslov: Plants
• Skrajšan naslov: Plants
• Založnik: MDPI
• ISSN: 2223-7747
• COBISS.SI-ID: 523345433

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Robinia pseudoacacia, navadna robinija, invazivne tujerodne rastline, kemična sestava, fitofarmacevtski izdelki, fenolne spojine, protimikrobna učinkovitost, citotoksična učinkovitost