20.500.12556/RUL-153017 Invasive alien species as a potential source of phytopharmaceuticals Phenolic composition and antimicrobial and cytotoxic activity of Robinia pseudoacacia L. leaf and flower extracts Black locust (Robinia pseudoacacia L.), an invasive tree in Europe, commonly known for its negative impact on biodiversity, is a rich source of phenolic compounds recognized in traditional medicine. Since the metabolite profile depends on the environment and climate, this study aimed to provide the first LC-MS phytochemical screening of the black locust from the Istria region (Croatia). The compounds were extracted from leaves and flowers with 70% ethanol and 80% methanol. Total phenolics (TP) and flavonoids (TF), as well as antioxidant capacity (AC) measured by ABTS (17.49–146.41 mg TE/g DW), DPPH (24.67–118.49 mg TE/g DW), and FRAP (7.38–77.53 mg TE/g DW) assays, were higher in leaf than in flower extracts. Higher TP and total non-flavonoid (TNF) values were displayed in ethanolic than in methanolic extracts. In total, 64 compounds were identified, of which flavonols (20) and hydroxycinnamic acid derivatives (15) were the most represented. Flavanols such as catechin dominated in leaf extracts, followed by flavonols, with kaempferol glucuronyl rhamnosyl hexosides as the main compound, respectively. Flower extracts had the highest share of flavones, followed by ellagitannins, with luteolin dirhamnosyl hexosides and vescalagin, respectively, being predominant. The extracts had good quorum sensing, biofilm formation prevention, and eradicating capacity. The results provided new insights into the phytochemical properties of R. pseudoacacia as the first step toward its potential pharmaceutical use. antioxidant antibacterial antibiofilm antifungal anti-quorum sensing black locust phenolics Robinia pseudoacacia navadna robinija invazivne tujerodne rastline kemična sestava fitofarmacevtski izdelki fenolne spojine protimikrobna učinkovitost citotoksična učinkovitost true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-14 12:41:16 2023-12-14 12:41:22 2024-04-24 09:54:34 0000-00-00 00:00:00 2023 0 0 23 str. iss. 14, art. 2715 Vol. 12 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.1/.7 2223-7747 177421571 plants-12-02715.pdf plants-12-02715.pdf 1 7428BE4C4548CDBD9B5355CB9FBBAA0F 8448a8cd87ea54ebe4b058cf8e9db22573b92b2aef491c1415bd1e5ff3d8b4f1 52683867-9a76-11ee-91f8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178800 https://www.mdpi.com/2223-7747/12/14/2715 1 b4ce8ca2-9a75-11ee-91f8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178799 Biotehniška fakulteta 0 0 0