izpis_h1_title_alt

Tandem mass spectrometry de novo sequencing of the skin defense peptides of the Central Slovenian agile frog Rana dalmatina
ID Samgina, Tatiana (Avtor), ID Vasileva, Irina D. (Avtor), ID Trebše, Polonca (Avtor), ID Torkar, Gregor (Avtor), ID Surin, Alexey K. (Avtor), ID Meng, Zhaowei (Avtor), ID Zubarev, Roman (Avtor), ID Lebedev, Albert T. (Avtor)

.pdfPDF - Predstavitvena datoteka, prenos (691,80 KB)
MD5: EE87CD2DF081BEAF5EEFA2A1716E796E
URLURL - Izvorni URL, za dostop obiščite https://www.mdpi.com/1420-3049/28/20/7118 Povezava se odpre v novem oknu

Izvleček
Peptides released on frogs’ skin in a stress situation represent their only weapon against micro-organisms and predators. Every species and even population of frog possesses its own peptidome being appropriate for their habitat. Skin peptides are considered potential pharmaceuticals, while the whole peptidome may be treated as a taxonomic characteristic of each particular population. Continuing the studies on frog peptides, here we report the peptidome composition of the Central Slovenian agile frog Rana dalmatina population. The detection and top-down de novo sequencing of the corresponding peptides was conducted exclusively by tandem mass spectrometry without using any chemical derivatization procedures. Collision-induced dissociation (CID), higher energy collision-induced dissociation (HCD), electron transfer dissociation (ETD) and combined MS3 method EThcD with stepwise increase of HCD energy were used for that purpose. MS/MS revealed the whole sequence of the detected peptides including differentiation between isomeric Leu/Ile, and the sequence portion hidden in the disulfide cycle. The array of the discovered peptide families (brevinins 1 and 2, melittin-related peptides (MRPs), temporins and bradykinin-related peptides (BRPs)) is quite similar to that of R. temporaria. Since the genome of this frog remains unknown, the obtained results were compared with the recently published transcriptome of R. dalmatina.

Jezik:Angleški jezik
Ključne besede:agile brown frog, membrane-active peptides, tandem mass spectrometry, top-down de novo sequencing, bradykinin-related peptides, BRP, EThcD
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:ZF - Zdravstvena fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Datum objave:16.10.2023
Leto izida:2023
Št. strani:14 str.
Številčenje:Vol. 28, iss. 20, art. 7118
PID:20.500.12556/RUL-151771 Povezava se odpre v novem oknu
UDK:543.51
ISSN pri članku:1420-3049
DOI:10.3390/molecules28207118 Povezava se odpre v novem oknu
COBISS.SI-ID:168907267 Povezava se odpre v novem oknu
Datum objave v RUL:19.10.2023
Število ogledov:727
Število prenosov:41
Metapodatki:XML DC-XML DC-RDF
:
Kopiraj citat
Objavi na:Bookmark and Share

Gradivo je del revije

Naslov:Molecules
Skrajšan naslov:Molecules
Založnik:MDPI
ISSN:1420-3049
COBISS.SI-ID:18462981 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:okretna rjava žaba, membransko aktivni peptidi, tandemska masna spektrometrija, de novo sekvenciranje od zgoraj navzdol, bradikininu sorodni peptidi, BRP, EThcD

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Nazaj