Tandem mass spectrometry de novo sequencing of the skin defense peptides of the Central Slovenian agile frog Rana dalmatina

Samgina, Tatiana (author), Vasileva, Irina D. (author), Trebše, Polonca (author), Torkar, Gregor (author), Surin, Alexey K. (author), Meng, Zhaowei (author), Zubarev, Roman (author), Lebedev, Albert T. (author)

Peptides released on frogs’ skin in a stress situation represent their only weapon against micro-organisms and predators. Every species and even population of frog possesses its own peptidome being appropriate for their habitat. Skin peptides are considered potential pharmaceuticals, while the whole peptidome may be treated as a taxonomic characteristic of each particular population. Continuing the studies on frog peptides, here we report the peptidome composition of the Central Slovenian agile frog Rana dalmatina population. The detection and top-down de novo sequencing of the corresponding peptides was conducted exclusively by tandem mass spectrometry without using any chemical derivatization procedures. Collision-induced dissociation (CID), higher energy collision-induced dissociation (HCD), electron transfer dissociation (ETD) and combined MS3 method EThcD with stepwise increase of HCD energy were used for that purpose. MS/MS revealed the whole sequence of the detected peptides including differentiation between isomeric Leu/Ile, and the sequence portion hidden in the disulfide cycle. The array of the discovered peptide families (brevinins 1 and 2, melittin-related peptides (MRPs), temporins and bradykinin-related peptides (BRPs)) is quite similar to that of R. temporaria. Since the genome of this frog remains unknown, the obtained results were compared with the recently published transcriptome of R. dalmatina.

• Language: English
• Keywords: agile brown frog, membrane-active peptides, tandem mass spectrometry, top-down de novo sequencing, bradykinin-related peptides, BRP, EThcD
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Publication date: 16.10.2023
• Year: 2023
• Number of pages: 14 str.
• Numbering: Vol. 28, iss. 20, art. 7118
• PID: 20.500.12556/RUL-151771
• UDC: 543.51
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules28207118
• COBISS.SI-ID: 168907267
• Publication date in RUL: 19.10.2023

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: okretna rjava žaba, membransko aktivni peptidi, tandemska masna spektrometrija, de novo sekvenciranje od zgoraj navzdol, bradikininu sorodni peptidi, BRP, EThcD