Volumetric absorptive microsampling for the therapeutic drug monitoring of psychiatric patients treated with cariprazine

Millán-Santiago, Jaime (avtor), Vitagliano, Rosalba (avtor), Mondella, Fortunata (avtor), Mandrioli, Roberto (avtor), Sardella, Roccaldo (avtor), Vovk, Tomaž (avtor), Lucena, Rafael (avtor), Cárdenas, Soledad (avtor), Boaron, Federico (avtor), Mercolini, Laura (avtor)

Psychiatric disorders are usually treated with antipsychotic agents belonging to different pharmacological and chemical classes, the most recent ones collectively known as “third-generation antipsychotics”, such as cariprazine, approved in 2015 for the treatment of patients affected by schizophrenia. For these patients, a frequent therapeutic drug monitoring (TDM) becomes essential to assess compliance and to optimise and personalise their therapy, also due to cariprazine interindividual variability and narrow therapeutic range. In this study, a bioanalytical method featuring miniaturised sampling and pretreatment was developed, based on volumetric absorptive microsampling (VAMS) for TDM of psychiatric patients under cariprazine treatment and compared to a classic, reference method based on fluid plasma analysis. Minimally invasive whole blood VAMS was coupled to an original instrumental method based on ultra-high performance liquid chromatography hyphenated to mass spectrometry (UHPLC-MS). A feasible and streamlined, yet reliable VAMS pretreatment protocol was carefully optimised and the VAMS-UHPLC-MS methodology was validated with satisfactory results in terms of linearity ($r^2$ > 0.9970 in the 1.5-100 ng/mL range), precision (%RSD ≤ 11.7), extraction yield (> 90.0%) and matrix effect (8.2≤ RE% ≤ 10.9%). Finally, the microsampling approach coupled to UHPLC-MS was successfully applied to the TDM of psychiatric patients treated with cariprazine and compared with standard fluid plasma analysis, providing reliable quali-quantitative results, and proving to be readily applicable to the clinical practice in TDM programs as a useful alternative to cariprazine plasma analysis. This is the first report of a successful microsampling application, and in particular the first report of VAMS application, for the TDM of cariprazine.

• Jezik: Angleški jezik
• Ključne besede: cariprazine, microsampling, therapeutic drug monitoring (TDM), volumetric absorptive microsampling (VAMS), microsampling (VAMS)
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 1-7
• Številčenje: Vol. 236, art. 115740
• PID: 20.500.12556/RUL-151577
• UDK: 615.214:616.89
• ISSN pri članku: 0731-7085
• DOI: 10.1016/j.jpba.2023.115740
• COBISS.SI-ID: 165592835
• Datum objave v RUL: 10.10.2023

Gradivo je del revije

• Naslov: Journal of pharmaceutical and biomedical analysis
• Skrajšan naslov: J. pharm. biomed. anal.
• Založnik: Elsevier B.V.
• ISSN: 0731-7085
• COBISS.SI-ID: 6557447

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kariprazin, mikrovzorčenje, terapevtsko spremljanje zdravil, volumetrično absorpcijsko mikrovzorčenje, antipsihotiki, psihiatrija

Projekti

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: Spanish Ministry of Science and Innovation
• Številka projekta: PID2020-112862RB-I00

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: Spanish Ministry of Science, Innovation and Universities
• Številka projekta: FPU19/01488