20.500.12556/RUL-151577 Volumetric absorptive microsampling for the therapeutic drug monitoring of psychiatric patients treated with cariprazine Psychiatric disorders are usually treated with antipsychotic agents belonging to different pharmacological and chemical classes, the most recent ones collectively known as “third-generation antipsychotics”, such as cariprazine, approved in 2015 for the treatment of patients affected by schizophrenia. For these patients, a frequent therapeutic drug monitoring (TDM) becomes essential to assess compliance and to optimise and personalise their therapy, also due to cariprazine interindividual variability and narrow therapeutic range. In this study, a bioanalytical method featuring miniaturised sampling and pretreatment was developed, based on volumetric absorptive microsampling (VAMS) for TDM of psychiatric patients under cariprazine treatment and compared to a classic, reference method based on fluid plasma analysis. Minimally invasive whole blood VAMS was coupled to an original instrumental method based on ultra-high performance liquid chromatography hyphenated to mass spectrometry (UHPLC-MS). A feasible and streamlined, yet reliable VAMS pretreatment protocol was carefully optimised and the VAMS-UHPLC-MS methodology was validated with satisfactory results in terms of linearity ($r^2$ > 0.9970 in the 1.5-100 ng/mL range), precision (%RSD ≤ 11.7), extraction yield (> 90.0%) and matrix effect (8.2≤ RE% ≤ 10.9%). Finally, the microsampling approach coupled to UHPLC-MS was successfully applied to the TDM of psychiatric patients treated with cariprazine and compared with standard fluid plasma analysis, providing reliable quali-quantitative results, and proving to be readily applicable to the clinical practice in TDM programs as a useful alternative to cariprazine plasma analysis. This is the first report of a successful microsampling application, and in particular the first report of VAMS application, for the TDM of cariprazine. cariprazine microsampling therapeutic drug monitoring (TDM) volumetric absorptive microsampling (VAMS) microsampling (VAMS) kariprazin mikrovzorčenje terapevtsko spremljanje zdravil volumetrično absorpcijsko mikrovzorčenje antipsihotiki psihiatrija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-10-10 09:55:51 2023-10-10 09:55:55 2023-10-11 03:33:50 0000-00-00 00:00:00 2023 0 0 Str. 1-7 art. 115740 Vol. 236 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.214:616.89 0731-7085 10.1016/j.jpba.2023.115740 165592835 1-s2.0-S0731708523005095-main.pdf 1-s2.0-S0731708523005095-main.pdf 1 748AC46A004ED5CBE78F04E7DA1202BE b757b35b799a5695c841421692870dba39597221082a481484c1d98b99050795 54639f67-6744-11ee-9430-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176571 https://www.sciencedirect.com/science/article/pii/S0731708523005095 1 71de017b-6742-11ee-9430-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176569 Fakulteta za farmacijo 0 0 0