The aim of the thesis was to evaluate the effect of extracts of four different cannabis chemotypes on different strains of S. aureus and S. epidermidis. The minimum inhibitory concentration (MIC) was determined by microdilution method and confirmed by the colony counting method on a solid medium. Cannabis extracts showed different MIC on individual strains, ranging from a minimum of 2.0±0 µl/mL to a maximum of 10.4±2.12 µl/mL, individual extracts had different effects on selected strains. In accordance with the established MICs, we aimed to determine the anti-adhesive activity of the four extracts on two of S. epidermidis type strains. We used the crystal violet staining method to verify the effect of the extracts on cell attachment to the microtitre plate. Concentrations of 0.5 MIC and 0.1 MIC were tested. The anti-adhesive activity was determined for E2 and MIC 0,1, E3 and MIC 0,5, E4 and MIC 0,5, E5 and MIC 0,1 for S. epidermidis ŽMJ 489 strain but not for S. epidermidis ŽMJ 465 strain. In view of the results, it can be concluded that cannabinoids have potential in pharmaceutical or medical applications to combat resistant strains of pathogenic microorganisms, although it should be noted that research in this field is still at an early stage.
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