eDNA provides a scaffold for autoaggregation of B. subtilis in bacterioplankton suspension

Dogša, Iztok (avtor), Kostanjšek, Rok (avtor), Stopar, David (avtor)

The self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and bacterial autoaggregate assembly were determined and related to the spatial autocorrelation of bacterial cells in dilute planktonic bacterial suspensions. The non-random distribution of cells was associated with an eDNA network, which stabilized the initial bacterial cell-cell aggregates. Upon the addition of DNase I, the aggregates were dispersed. The release of eDNA during cell lysis allows for the entrapment of bacterial drifters at a radius several times the size of the dying bacteria. The size of bacterial aggregates increased from 2 to about 100 μm in diameter in dilute bacterial suspensions. The results suggest that B. subtilis cells form previously unnoticed continuum of autoaggregate structures during planktonic growth.

• Jezik: Angleški jezik
• Ključne besede: autoaggregates, B. subtilis, plankton, eDNA, biofilm
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 15 str.
• Številčenje: Vol. 11, iss. 2, art. 332
• PID: 20.500.12556/RUL-145742
• UDK: 579.25:579.852.11
• ISSN pri članku: 2076-2607
• DOI: 10.3390/microorganisms11020332
• COBISS.SI-ID: 142263043
• Datum objave v RUL: 11.05.2023

Gradivo je del revije

• Naslov: Microorganisms
• Skrajšan naslov: Microorganisms
• Založnik: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: avtoagregacija, eDNA, bakterijski plankton, Bacillus subtilis, biofilm