20.500.12556/RUL-145742 eDNA provides a scaffold for autoaggregation of B. subtilis in bacterioplankton suspension The self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and bacterial autoaggregate assembly were determined and related to the spatial autocorrelation of bacterial cells in dilute planktonic bacterial suspensions. The non-random distribution of cells was associated with an eDNA network, which stabilized the initial bacterial cell-cell aggregates. Upon the addition of DNase I, the aggregates were dispersed. The release of eDNA during cell lysis allows for the entrapment of bacterial drifters at a radius several times the size of the dying bacteria. The size of bacterial aggregates increased from 2 to about 100 μm in diameter in dilute bacterial suspensions. The results suggest that B. subtilis cells form previously unnoticed continuum of autoaggregate structures during planktonic growth. autoaggregates B. subtilis plankton eDNA biofilm avtoagregacija eDNA bakterijski plankton Bacillus subtilis biofilm true false true Angleški jezik Slovenski jezik Članek v reviji 2023-05-11 11:43:46 2023-05-11 11:43:47 2023-05-12 03:35:57 0000-00-00 00:00:00 2023 0 0 15 str. iss. 2, art. 332 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.25:579.852.11 2076-2607 10.3390/microorganisms11020332 142263043 RAZ_Dogsa_Iztok_2023.pdf RAZ_Dogsa_Iztok_2023.pdf 1 CEF16442C5A77218A481C4ADAE3ED6D6 5bea28bf200b9b7640fd012cb1c00faaf1d2fd1c3557e2854ff07dab24ae06ca aff40d3e-efe1-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168747 https://www.mdpi.com/2076-2607/11/2/332 1 163f11c4-efe0-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168745 Biotehniška fakulteta 0 0 0