Bacillus subtilis PS-216 spores supplemented in broiler chicken drinking water reduce Campylobacter jejuni colonization and increase weight gain

Šimunović, Katarina (avtor), Sahin, Orhan (avtor), Erega, Andi (avtor), Štefanič, Polonca (avtor), Zhang, Qijing (avtor), Mandić-Mulec, Ines (avtor), Smole Možina, Sonja (avtor), Klančnik, Anja (avtor)

Campylobacter jejuni is the leading cause of bacterial gastroenteritis, or campylobacteriosis, in humans worldwide, and poultry serves as a major source of infection. To reduce the risk associated with C. jejuni transmission via poultry meat, effective interventions during poultry production are needed, and the use of probiotics is a promising approach. In this study, 15 Bacillus subtilis strains were initially screened for their anti-Campylobacter activities. B. subtilis PS-216 strain demonstrated the best anti-Campylobacter activity against 15 C. jejuni isolates when examined using in vitro co-cultures. To evaluate the suitability of B. subtilis PS-216 for probiotic use, its susceptibility to eight clinically important antimicrobials and simulated gastric conditions was investigated. B. subtilis PS-216 was sensitive to all of the tested antibiotics. Although vegetative cells were sensitive to gastric conditions, B. subtilis PS-216 spores were highly resistant. We further evaluated the use of a B. subtilis PS-216 spore preparation (2.5 × 10$^6$ CFU/mL water) to prevent and/or reduce C. jejuni colonization in broiler chickens in vivo. Compared to the untreated group, significantly lower Campylobacter counts were detected in caeca of broilers continuously treated with B. subtilis PS-216 spores in their drinking water. Furthermore, broilers continuously treated with B. subtilis PS-216 spores showed improved weight gain, compared to the control group. Together, these results demonstrate the potential of B. subtilis PS-216 for use in poultry to reduce C. jejuni colonization and improve weight gain.

• Jezik: Angleški jezik
• Ključne besede: Campylobacter jejuni, Bacillus subtilis, probiotics, alternative to antibiotics, spore-containing drinking water, broiler chicken
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 11 str.
• Številčenje: Vol. 13, art. 910616
• PID: 20.500.12556/RUL-145323
• UDK: 579.22/.26:579.67
• ISSN pri članku: 1664-302X
• DOI: 10.3389/fmicb.2022.910616
• COBISS.SI-ID: 115015683
• Datum objave v RUL: 17.04.2023

Gradivo je del revije

• Naslov: Frontiers in microbiology
• Skrajšan naslov: Front. microbiol.
• Založnik: Frontiers Research Foundation
• ISSN: 1664-302X
• COBISS.SI-ID: 4146296

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: probiotiki, inhibicija rasti, sporogene bakterije, perutnina